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    Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance


    Maurer, Elisabeth and Browne, Niall and Surlis, Carla and Jukic, Emina and Moser, Patrizia and Kavanagh, Kevin and Lass-Flörl, Cornelia and Binder, Ulrike (2015) Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance. Virulence, 6 (6). pp. 591-598. ISSN 2150-5608

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    Abstract

    The aim of this study was to investigate if the alternative in vivo model Galleria mellonella can be used (i) to determine differences in pathogenicity of amphotericin B (AMB) resistant and susceptible A. terreus isolates, (ii) to evaluate AMB efficacy in vivo (iii) and to correlate outcome to in vitro susceptibility data. Larvae were infected with 2 A. terreus AMB resistant (ATR) and 3 AMB susceptible (ATS) isolates and survival rates were correlated to physiological attributes and killing ability of larval haemocytes. Additionally, infected larvae were treated with different concentrations of L-AMB. Haemocyte density were ascertained to evaluate the influence of L-AMB on the larval immune cells. Larvae were sensitive to A. terreus infection in an inoculum-size and temperature dependent manner. In vitro susceptibility to L-AMB correlated with in vivo outcome of antifungal treatment, defining an AMB susceptible strain cluster of A. terreus. Susceptibility to L-AMB increased virulence potential in the larval model, but this increase was also in accordance with faster growth and less damage caused by larval haemocytes. L-AMB treatment primed the larval immune response by increasing haemocyte density. G. mellonella provides a convenient model for the in vivo screening of A. terreus virulence and treatment options, contributing to the generation of a hypothesis that can be further tested in refined experiments in mammalian models.

    Item Type: Article
    Additional Information: Cite as: Elisabeth Maurer, Niall Browne, Carla Surlis, Emina Jukic, Patrizia Moser, Kevin Kavanagh, Cornelia Lass-Flörl & Ulrike Binder (2015) Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance, Virulence, 6:6, 591-598, DOI: 10.1080/21505594.2015.1045183
    Keywords: amphotericin B resistant and susceptible A. terreus strain cluster; Galleria mellonella; invertebrate in vivo model;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11101
    Identification Number: https://doi.org/10.1080/21505594.2015.1045183
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 25 Sep 2019 16:03
    Journal or Publication Title: Virulence
    Publisher: Taylor & Francis Open
    Refereed: Yes
    URI:

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