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    Monoclonal antibodies directed against extracellular matrix proteins reduce the adherence of Candida albicans to HEp-2 cells


    Cotter, Gary and Wheedle, Roisin and Kavanagh, Kevin (1998) Monoclonal antibodies directed against extracellular matrix proteins reduce the adherence of Candida albicans to HEp-2 cells. Mycopathologia, 141 (3). pp. 137-142. ISSN 1573-0832

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    Abstract

    The presence of the extracellular matrix (ECM) proteins collagen types I and IV, laminin and fibronectin on the surface of HEp-2 cells was confirmed by flow cytometry using monoclonal antibodies. Monoclonal antibodies directed against these ECM proteins reduced the adherence of C. albicans ATCC 44990 to HEp-2 cells, the greatest reductions being evident in assays which incorporated anti-collagen type IV monoclonal antibody. The ability of sugaramines to inhibit the adherence of C. albicans to a variety of cell types has been demonstrated previously and the most significant reduction in C. albicans – HEp-2 adherence was in assays which incorporated 0.2M galactosamine. The combination of anti-collagen IV monoclonal antibody and galactosamine reduced the adherence of C. albicans to HEp-2 cells by approximately 70% (p < 0.05).

    Item Type: Article
    Additional Information: Cite as: Cotter, G., Weedle, R. & Kavanagh, K. Mycopathologia (1998) 141: 137. https://doi.org/10.1023/A:1006940203962
    Keywords: Adherence; Candida; Extracellular matrix; HEp-2;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11120
    Identification Number: https://doi.org/10.1023/A:1006940203962
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 03 Oct 2019 14:26
    Journal or Publication Title: Mycopathologia
    Publisher: Kluwer Academic Publishers
    Refereed: Yes
    URI:

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