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    Enhanced intraspecific protoplast fusion in yeast


    Kavanagh, Kevin and Walsh, M. and Whittaker, Peter A. (1991) Enhanced intraspecific protoplast fusion in yeast. FEMS Microbiology Letters, 81 (3). pp. 283-286. ISSN 0378-1097

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    Abstract

    Intraspecific hybrid production from the polyethylene glycol induced fusion of yeast protoplasts was greatly increased when calcium propionate was included as the source of the requisite Ca2+. The use of calcium propionate, as opposed to the more commonly employed calcium chloride, resulted in substantially greater yields of hybrids from intraspecific fusions of protoplasts of Saccharomyces cerevisiae and Candida albicans. It is postulated that the ability of calcium propionate to enhance the fusion frequency is due to the anion binding to the etheric oxygen of PEG and potentiating the fusogenicity of the polymer.

    Item Type: Article
    Keywords: Protoplast fusion; Yeast; Candida albicans; Saccharomyces cerevisiae;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11127
    Identification Number: https://doi.org/10.1111/j.1574-6968.1991.tb04773.x
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 04 Oct 2019 13:28
    Journal or Publication Title: FEMS Microbiology Letters
    Publisher: Elsevier B.V.
    Refereed: Yes
    URI:

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