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    Fungal proteomics: from identification to function


    Doyle, Sean (2011) Fungal proteomics: from identification to function. FEMS Microbiology Letters, 321 (1). pp. 1-9. ISSN 0378-1097

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    Abstract

    Some fungi cause disease in humans and plants, while others have demonstrable potential for the control of insect pests. In addition, fungi are also a rich reservoir of therapeutic metabolites and industrially useful enzymes. Detailed analysis of fungal biochemistry is now enabled by multiple technologies including protein mass spectrometry, genome and transcriptome sequencing and advances in bioinformatics. Yet, the assignment of function to fungal proteins, encoded either by in silico annotated, or unannotated genes, remains problematic. The purpose of this review is to describe the strategies used by many researchers to reveal protein function in fungi, and more importantly, to consolidate the nomenclature of ‘unknown function protein’ as opposed to ‘hypothetical protein’– once any protein has been identified by protein mass spectrometry. A combination of approaches including comparative proteomics, pathogen-induced protein expression and immunoproteomics are outlined, which, when used in combination with a variety of other techniques (e.g. functional genomics, microarray analysis, immunochemical and infection model systems), appear to yield comprehensive and definitive information on protein function in fungi. The relative advantages of proteomic, as opposed to transcriptomic-only, analyses are also described. In the future, combined high-throughput, quantitative proteomics, allied to transcriptomic sequencing, are set to reveal much about protein function in fungi.

    Item Type: Article
    Additional Information: Cite as: Sean Doyle, Fungal proteomics: from identification to function, FEMS Microbiology Letters, Volume 321, Issue 1, August 2011, Pages 1–9, https://doi.org/10.1111/j.1574-6968.2011.02292.x
    Keywords: fungal proteomics; protein mass spectrometry; MALDI-ToF; LC-MS; Aspergillus fumigatus; gliotoxin;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11218
    Identification Number: https://doi.org/10.1111/j.1574-6968.2011.02292.x
    Depositing User: Dr. Sean Doyle
    Date Deposited: 10 Oct 2019 14:59
    Journal or Publication Title: FEMS Microbiology Letters
    Publisher: Blackwell Publishing Ltd
    Refereed: Yes
    Funders: Higher Education Authority (HEA), Enterprise Ireland (EI), Irish Research Council for Science, Engineering and Technology (IRCSET)
    URI:

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