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    Evaluation of an Enzyme-Immunometric Assay for Serum α-Glutathione S-Transferase


    Rees, Gareth W. and Trull, Andrew K. and Doyle, Sean (1995) Evaluation of an Enzyme-Immunometric Assay for Serum α-Glutathione S-Transferase. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 32 (6). pp. 575-583. ISSN 1758-1001

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    Abstract

    A commercially available enzyme-immunometric assay for serum α-glutathione S-transferase (GST) was evaluated. Endogenous serum α-GST diluted linearly within the calibration range. However, we recommend that the sample and second antibody reagent are always added sequentially in the assay to avoid hook effect. Between-assay variability was below 7% across the calibration range and the upper limit of the reference range in adults (n = 219) was 11 · 4 μg/L. Within-individual variability in serum α-GST concentrations measured over a 4–6 week period in 4 healthy adults was small. Serum α-GST concentrations did not change significantly 6 h after a therapeutic dose of paracetamol. Studies in two patients after liver transplantation showed that serum α-GST is a better discriminant of acute changes in liver function than conventional tests. Serum α-GST concentrations were unaffected by gross muscle damage, extra-hepatic inflammation, or haemolysis and thus appear to be more liver specific than transaminase activities. The effect of renal impairment on serum α-GST concentrations requires further investigation.

    Item Type: Article
    Additional Information: Cite as: Rees, G. W., Trull, A. K., & Doyle, S. (1995). Evaluation of an Enzyme-Immunometric Assay for Serum α-Glutathione S-Transferase. Annals of Clinical Biochemistry, 32(6), 575–583. https://doi.org/10.1177/000456329503200610
    Keywords: serum α-GST; enzyme-immunometric assay; method validation; liver function; tissue specificity; ALT; AST;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11221
    Identification Number: https://doi.org/10.1177/000456329503200610
    Depositing User: Dr. Sean Doyle
    Date Deposited: 10 Oct 2019 16:32
    Journal or Publication Title: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
    Publisher: SAGE Publications
    Refereed: Yes
    URI:

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