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    Alternative Translocation Breakpoint Cluster Region 5' to BCL-6 in B-cell Non-Hodgkin’s Lymphoma


    Butler, Marion P. and Iida, Shinsuke and Capello, Daniela and Rossi, Davide and Rao, Pulivarthi H. and Nallasivam, Palanisamy and Louie, Diane C. and Chaganti, Seeta and Au, Thomas and Gascoyne, Randy D. and Gaidano, Gianluca and Chaganti, Raju S. K. and Dalla-Favera, Riccardo (2002) Alternative Translocation Breakpoint Cluster Region 5' to BCL-6 in B-cell Non-Hodgkin’s Lymphoma. Cancer Research, 62. pp. 4089-4094. ISSN 0008-5472

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    Abstract

    Chromosomal translocations involving band 3q27 with various different partner chromosomes represent a recurrent cytogenetic abnormality in B-cell non-Hodgkin’s lymphoma. In a fraction of these translocations, the chromosomal breakpoint is located within the 5' noncoding region of the BCL-6 proto-oncogene where the BCL-6 major breakpoint region (MBR) maps. As a result of the translocation, BCL-6 expression is deregulated by promoter substitution. However, between 30 and 50% of lymphomas with cytogenetically detectable translocations affecting band 3q27 retain a germ-line configuration at the BCL-6 locus. To identify possible additional breakpoint clusters within 3q27, we cloned a t(3;14)(q27;q32) lymphoma without MBR rearrangement and found a novel breakpoint site located between 245 and 285 kb 5' to BCL-6. Breakpoints within this newly described region, which we called the alternative breakpoint region (ABR), were found to be recurrent in lymphomas carrying t(3q27) chromosomal translocations but devoid of BCL-6 MBR rearrangements. Comparative analysis of multiple lymphomas carrying rearrangements within the ABR showed that the breakpoints cluster within a 20-kb distance. Translocations involving the ABR may juxtapose BCL-6 to distantly acting, heterologous transcriptional regulatory elements which cause deregulation of the proto-oncogene. The identification of BCL-6 ABR provides new tools for the diagnosis of lymphomas carrying aberrations at 3q27 and deregulated BCL-6 genes.

    Item Type: Article
    Keywords: Alternative; Translocation; Breakpoint Cluster; Region 5′ to BCL-6; B-cell; Non-Hodgkin’s Lymphoma;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 12354
    Depositing User: Marion Butler
    Date Deposited: 04 Feb 2020 15:54
    Journal or Publication Title: Cancer Research
    Publisher: American Association for Cancer Research
    Refereed: Yes
    URI:

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