Harris, J. Kirk and Fang, R. and Wagner, Brandie D. and Choe, Ha Na and Kelly, Caleb J. and Schroeder, Shauna and Moore, Wendy and Stevens, Mark J. and Yeckes, Alyson and Amsden, Katie and Kagalwalla, Amir F. and Zalewski, Angelika and Hirano, Ikuo and Gonsalves, Nirmala and Henry, Lauren N. and Masterson, Joanne C. and Robertson, Charles E. and Leung, Donald Y. and Pace, Norman R. and Ackerman, Steven J. and Furuta, Glenn T. and Fillon, Sophie A. (2015) Esophageal Microbiome in Eosinophilic Esophagitis. PLoS ONE, 10 (5). e0128346. ISSN 1932-6203

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Abstract

Objective: The microbiome has been implicated in the pathogenesis of a number of allergic and inflammatory diseases. The mucosa affected by eosinophilic esophagitis (EoE) is composed of a stratified squamous epithelia and contains intraepithelial eosinophils. To date, no studies have identified the esophageal microbiome in patients with EoE or the impact of treatment on these organisms. The aim of this study was to identify the esophageal microbiome in EoE and determine whether treatments change this profile. We hypothesized that clinically relevant alterations in bacterial populations are present in different forms of esophagitis. Design: In this prospective study, secretions from the esophageal mucosa were collected from children and adults with EoE, Gastroesophageal Reflux Disease (GERD) and normal mucosa using the Esophageal String Test (EST). Bacterial load was determined using quantitative PCR. Bacterial communities, determined by 16S rRNA gene amplification and 454 pyrosequencing, were compared between health and disease. Results: Samples from a total of 70 children and adult subjects were examined. Bacterial load was increased in both EoE and GERD relative to normal subjects. In subjects with EoE, load was increased regardless of treatment status or degree of mucosal eosinophilia compared with normal. Haemophilus was significantly increased in untreated EoE subjects as compared with normal subjects. Streptococcus was decreased in GERD subjects on proton pump inhibition as compared with normal subjects. Conclusions: Diseases associated with mucosal eosinophilia are characterized by a different microbiome from that found in the normal mucosa. Microbiota may contribute to esophageal inflammation in EoE and GERD.

Item Type:	Article
Additional Information:	© 2015 Harris et al. This is an open access article distributed under the terms of the Creative Commons Attribution License https://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Cite as: Harris JK, Fang R, Wagner BD, Choe HN,Kelly CJ, Schroeder S, et al. (2015) EsophagealMicrobiome in Eosinophilic Esophagitis. PLoS ONE10(5): e0128346. doi:10.1371/journal.pone.0128346
Keywords:	Esophageal Microbiome; Eosinophilic Esophagitis;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	12485
Identification Number:	https://doi.org/10.1371/journal.pone.0128346
Depositing User:	Joanne Masterson
Date Deposited:	26 Feb 2020 17:00
Journal or Publication Title:	PLoS ONE
Publisher:	Public Library of Science
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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