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    Esophageal human β-defensin expression in eosinophilic esophagitis.


    Schroeder, Shauna and Robinson, Zachary and Masterson, Joanne C. and Hosford, Lindsay and Moore, Wendy and Pan, Zhaoxing and Harris, Rachel and Souza, Rhonda F. and Spechler, Stuart Jon and Fillon, Sophie A. and Furuta, Glenn T. (2013) Esophageal human β-defensin expression in eosinophilic esophagitis. Pediatric Research, 73 (5). pp. 647-654. ISSN 1530-0447

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    Abstract

    Background: Defensins are antimicrobial peptides expressed on mucosal surfaces that contribute to maintaining intestinal homeostasis by providing innate defense mechanisms for the epithelia. Defensin expression is altered in a number of diseases that affect mucosal surfaces, such as atopic dermatitis, allergic rhinitis, and inflammatory bowel disease. Similar to atopic dermatitis, eosinophilic esophagitis (EoE) is a chronic disease in which the squamous epithelial surface is affected by a similar TH2 microenvironment and eosinophilpredominant inflammation. Therefore, we hypothesized that defensin expression would be decreased in EoE. Methods: To address this, we measured defensin expression invitro in cell lines derived from patients with EoE (EoE1-T) or gastroesophageal reflux disease (GERD) (NES-G4T cells) and ex vivo in esophageal mucosal biopsy samples from children with EoE or GERD and control children without esophageal disease. Results: Interleukin-5 induced a decrease in human β-defensin (hBD) -1 and hBD3 expression in EoE1-T but not in NES-G4T cells. Compared with esophageal biopsy specimens from GERD and control children, specimens from EoE pediatric patients revealed a significant decrease in mRNA and protein expression for hBD1 and hBD3. Conclusion: Diminished expression of hBD1 and hBD3 may make the esophageal epithelium more susceptible to the development and/or perpetuation of EoE.

    Item Type: Article
    Keywords: Esophageal; human β-defensin; expression; eosinophilic esophagitis;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 12492
    Identification Number: https://doi.org/10.1038/pr.2013.23
    Depositing User: Joanne Masterson
    Date Deposited: 27 Feb 2020 15:02
    Journal or Publication Title: Pediatric Research
    Publisher: Nature Publishing Group
    Refereed: Yes
    URI:

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