Fillon, Sophie A. and Harris, J. Kirk and Wagner, Brandie D. and Kelly, Caleb J. and Stevens, Mark J. and Moore, Wendy and Fang, Rui and Schroeder, Shauna and Masterson, Joanne C. and Robertson, Charles E. and Pace, Norman R. and Ackerman, Steven J. and Furuta, Glenn T. (2012) Novel Device to Sample the Esophageal Microbiome— The Esophageal String Test. PLoS ONE, 7 (9). e42938. ISSN 1932-6203

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Abstract

A growing number of studies implicate the microbiome in the pathogenesis of intestinal inflammation. Previous work has shown that adults with esophagitis related to gastroesophageal reflux disease have altered esophageal microbiota compared to those who do not have esophagitis. In these studies, sampling of the esophageal microbiome was accomplished by isolating DNA from esophageal biopsies obtained at the time of upper endoscopy. The aim of the current study was to identify the esophageal microbiome in pediatric individuals with normal esophageal mucosa using a minimally invasive, capsule-based string technology, the EnterotestTM. We used the proximal segment of the Enterotest string to sample the esophagus, and term this the ‘‘Esophageal String Test’’ (EST). We hypothesized that the less invasive EST would capture mucosal adherent bacteria present in the esophagus in a similar fashion as mucosal biopsy. EST samples and mucosal biopsies were collected from children with no esophageal inflammation (n = 15) and their microbiome composition determined by 16S rRNA gene sequencing. Microbiota from esophageal biopsies and ESTs produced nearly identical profiles of bacterial genera and were different from the bacterial contents of samples collected from the nasal and oral cavity. We conclude that the minimally invasive EST can serve as a useful device for study of the esophageal microbiome.

Item Type:	Article
Additional Information:	©2012 Fillon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Cite as: Fillon SA, Harris JK, Wagner BD, Kelly CJ, Stevens MJ, et al. (2012) Novel Device to Sample the Esophageal Microbiome—The Esophageal StringTest. PLoS ONE 7(9): e42938. doi:10.1371/journal.pone.0042938
Keywords:	Novel Device; Sample; Esophageal Microbiome; Esophageal String Test;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	12494
Identification Number:	https://doi.org/10.1371/journal.pone.0042938
Depositing User:	Joanne Masterson
Date Deposited:	27 Feb 2020 15:18
Journal or Publication Title:	PLoS ONE
Publisher:	Public Library of Science
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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