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    A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling


    Fuku, Xolile and Singh, Baljit and Ajayi, Rachel F. and Jijana, Abongile N. and Baker, Priscilla and Dempsey, Eithne and Iwuoha, Emmanuel (2015) A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling. Analytical Methods, 7 (15). pp. 6114-6124. ISSN 1759-9660

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    Abstract

    Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH2-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng−1 mL−1 and 0.2 pg mL−1 Her2/neu oncogene, respectively.

    Item Type: Article
    Keywords: Breast Cancer; Immunohistochemistry; Fabrication; Metastasis; Expression; Biomarkers; Pathology; Electrode; Toxicity; Invasion;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 12523
    Identification Number: https://doi.org/10.1039/c5ay00962f
    Depositing User: Eithne Dempsey
    Date Deposited: 05 Mar 2020 16:51
    Journal or Publication Title: Analytical Methods
    Publisher: Royal Society of Chemistry
    Refereed: Yes
    URI:

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