Robinson, Mark W. and Swann, R and Sigruener, A. and Barclay, S. T. and Mills, P. R. and McLauchlan, J. and Patel, A. H. (2015) Elevated interferon‐stimulated gene transcription in peripheral blood mononuclear cells occurs in patients infected with genotype 1 but not genotype 3 hepatitis C virus. Journal of Viral Hepatitis, 22. pp. 384-390. ISSN 1352-0504

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Abstract

Hepatitis C virus (HCV) can be classified into seven distinct genotypes that are associated with differing pathologies and respond differently to antiviral therapy. In the UK, genotype 1 and 3 are present in approximately equal proportions. Chronic infection with HCV genotype 3 is associated with increased liver steatosis and reduced peripheral total cholesterol levels, which potentially influences peripheral immune responses. To understand these differences, we investigated host gene transcription in peripheral blood mononuclear cells by microarray and quantitative PCR in patients with genotype 1 (n = 22) or genotype 3 infection (n = 22) and matched healthy controls (n = 15). Enrichment of genes involved in immune response and inflammatory pathways were present in patients infected with HCV genotype 1; however, no differences in genes involved in lipid or cholesterol metabolism were detected. This genotype‐specific induction of genes is unrelated to IL28B genotype or previous treatment failure. Our data support the hypothesis that genotype 1 infection drives a skewed Type I interferon response and provides a foundation for future investigations into the host–pathogen interactions that underlie the genotype‐specific clinical outcomes of chronic HCV infection.

Item Type:	Article
Keywords:	genotype; HCV; IFN; interferon-stimulated genes; transcriptomics;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	12660
Identification Number:	https://doi.org/10.1111/jvh.12310
Depositing User:	Mark Robinson
Date Deposited:	30 Mar 2020 10:37
Journal or Publication Title:	Journal of Viral Hepatitis
Publisher:	Wiley
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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