MURAL - Maynooth University Research Archive Library



    Elevated interferon‐stimulated gene transcription in peripheral blood mononuclear cells occurs in patients infected with genotype 1 but not genotype 3 hepatitis C virus.


    Robinson, Mark W. and Swann, R and Sigruener, A. and Barclay, S. T. and Mills, P. R. and McLauchlan, J. and Patel, A. H. (2015) Elevated interferon‐stimulated gene transcription in peripheral blood mononuclear cells occurs in patients infected with genotype 1 but not genotype 3 hepatitis C virus. Journal of Viral Hepatitis, 22. pp. 384-390. ISSN 1352-0504

    [img]
    Preview
    Download (499kB) | Preview


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    Hepatitis C virus (HCV) can be classified into seven distinct genotypes that are associated with differing pathologies and respond differently to antiviral therapy. In the UK, genotype 1 and 3 are present in approximately equal proportions. Chronic infection with HCV genotype 3 is associated with increased liver steatosis and reduced peripheral total cholesterol levels, which potentially influences peripheral immune responses. To understand these differences, we investigated host gene transcription in peripheral blood mononuclear cells by microarray and quantitative PCR in patients with genotype 1 (n = 22) or genotype 3 infection (n = 22) and matched healthy controls (n = 15). Enrichment of genes involved in immune response and inflammatory pathways were present in patients infected with HCV genotype 1; however, no differences in genes involved in lipid or cholesterol metabolism were detected. This genotype‐specific induction of genes is unrelated to IL28B genotype or previous treatment failure. Our data support the hypothesis that genotype 1 infection drives a skewed Type I interferon response and provides a foundation for future investigations into the host–pathogen interactions that underlie the genotype‐specific clinical outcomes of chronic HCV infection.

    Item Type: Article
    Keywords: genotype; HCV; IFN; interferon-stimulated genes; transcriptomics;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 12660
    Identification Number: https://doi.org/10.1111/jvh.12310
    Depositing User: Mark Robinson
    Date Deposited: 30 Mar 2020 10:37
    Journal or Publication Title: Journal of Viral Hepatitis
    Publisher: Wiley
    Refereed: Yes
    URI:

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year