Schroeder, Martina and Kroeger, Karen M. and Hans-Dieter, Volk and Eidne, Karin A. and Grutz, Gerald (2004) Preassociation of nonactivated STAT3 molecules demonstrated in living cells using bioluminescence resonance energy transfer: a new model of STAT activation? Journal Of Leukocyte Biology, 75. pp. 792-797. ISSN 1938-3673

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Abstract

Signal transducers and activators of transcription (STATs) are crucial molecules in cytokine signaling. In the conventional model of STAT activation, STAT molecules are recruited from a latent pool of cytoplasmic monomers to the activated cytokine receptor. After binding to the receptor, they get tyrosine-phosphorylated, dissociate from the receptor, and translocate to the nucleus as activation-induced dimers. Recently, severa publications questioned this model of STAT activation and showed the existence of preassociated STAT molecules before activation. We were able to demonstrate the existence of these preassociated STAT3 molecules in living mammalian cells using bioluminescence resonance energy transfer. Our results support the new hypothesis that STAT molecules exist in the cytoplasm as dimers or multimers and point to an activation induced change in STAT3 conformation. Therefore, we propose a new model of STAT activation and discuss a hypothetical structure of “cytoplasmic” STAT dimers as opposed to the known “activation-induced” dimer.

Item Type:	Article
Keywords:	cytokine; signal transduction; dimerization signal transducer and activator of transcription (STAT); energy transfer; BRET;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	12666
Identification Number:	https://doi.org/10.1189/ jlb.1003496
Depositing User:	Martina Schroeder
Date Deposited:	31 Mar 2020 12:37
Journal or Publication Title:	Journal Of Leukocyte Biology
Publisher:	Wiley Online Library
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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