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    Preassociation of nonactivated STAT3 molecules demonstrated in living cells using bioluminescence resonance energy transfer: a new model of STAT activation?


    Schroeder, Martina and Kroeger, Karen M. and Hans-Dieter, Volk and Eidne, Karin A. and Grutz, Gerald (2004) Preassociation of nonactivated STAT3 molecules demonstrated in living cells using bioluminescence resonance energy transfer: a new model of STAT activation? Journal Of Leukocyte Biology, 75. pp. 792-797. ISSN 1938-3673

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    Abstract

    Signal transducers and activators of transcription (STATs) are crucial molecules in cytokine signaling. In the conventional model of STAT activation, STAT molecules are recruited from a latent pool of cytoplasmic monomers to the activated cytokine receptor. After binding to the receptor, they get tyrosine-phosphorylated, dissociate from the receptor, and translocate to the nucleus as activation-induced dimers. Recently, severa publications questioned this model of STAT activation and showed the existence of preassociated STAT molecules before activation. We were able to demonstrate the existence of these preassociated STAT3 molecules in living mammalian cells using bioluminescence resonance energy transfer. Our results support the new hypothesis that STAT molecules exist in the cytoplasm as dimers or multimers and point to an activation induced change in STAT3 conformation. Therefore, we propose a new model of STAT activation and discuss a hypothetical structure of “cytoplasmic” STAT dimers as opposed to the known “activation-induced” dimer.

    Item Type: Article
    Keywords: cytokine; signal transduction; dimerization signal transducer and activator of transcription (STAT); energy transfer; BRET;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 12666
    Identification Number: https://doi.org/10.1189/ jlb.1003496
    Depositing User: Martina Schroeder
    Date Deposited: 31 Mar 2020 12:37
    Journal or Publication Title: Journal Of Leukocyte Biology
    Publisher: Wiley Online Library
    Refereed: Yes
    URI:

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