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    Comparative Genomic and Proteomic Analyses of Three Widespread Phytophthora Species: Phytophthora chlamydospora, Phytophthora gonapodyides and Phytophthora pseudosyringae


    McGowan, Jamie and O'Hanlon, Richard and Owens, Rebecca A. and Fitzpatrick, David A. (2020) Comparative Genomic and Proteomic Analyses of Three Widespread Phytophthora Species: Phytophthora chlamydospora, Phytophthora gonapodyides and Phytophthora pseudosyringae. Microorganisms, 8 (5). ISSN 2076-2607

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    Abstract

    The genus includes some of the most devastating plant pathogens. Here we report draft genome sequences for three ubiquitous species-, , and . is an important forest pathogen that is abundant in Europe and North America. and are globally widespread species often associated with aquatic habitats. They are both regarded as opportunistic plant pathogens. The three sequenced genomes range in size from 45 Mb to 61 Mb. Similar to other oomycete species, tandem gene duplication appears to have played an important role in the expansion of effector arsenals. Comparative analysis of carbohydrate-active enzymes (CAZymes) across 44 oomycete genomes indicates that oomycete lifestyles may be linked to CAZyme repertoires. The mitochondrial genome sequence of each species was also determined, and their gene content and genome structure were compared. Using mass spectrometry, we characterised the extracellular proteome of each species and identified large numbers of proteins putatively involved in pathogenicity and osmotrophy. The mycelial proteome of each species was also characterised using mass spectrometry. In total, the expression of approximately 3000 genes per species was validated at the protein level. These genome resources will be valuable for future studies to understand the behaviour of these three widespread species.

    Item Type: Article
    Keywords: comparative genomics; effectors; extracellular proteome; secretome; genome evolution;mass spectrometry; phylogenomics; plant pathogen; phytophthora proteomics;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 13236
    Depositing User: David Fitzpatrick
    Date Deposited: 17 Sep 2020 10:21
    Journal or Publication Title: Microorganisms
    Publisher: MDPI
    Refereed: Yes
    URI:

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