Bauer, Ingo and Gross, Silke and Merschak, Petra and Kremser, Leopold and Karahoda, Betim and Bayram, Ozlem Sarikaya and Abt, Beate and Binder, Ulrike and Gsaller, Fabio and Lindner, Herbert and Bayram, Ozgur and Brosch, Gerald and Graessle, Stefan (2020) RcLS2F – A Novel Fungal Class 1 KDAC Co-repressor Complex in Aspergillus nidulans. Frontiers in Microbiology, 11 (43). ISSN 1664-302X

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Abstract

The fungal class 1 lysine deacetylase (KDAC) RpdA is a promising target for prevention and treatment of invasive fungal infection. RpdA is essential for survival of the most common air-borne mold pathogen Aspergillus fumigatus and the model organism Aspergillus nidulans. In A. nidulans, RpdA depletion induced production of previously unknown small bioactive substances. As known from yeasts and mammals, class 1 KDACs act as components of multimeric protein complexes, which previously was indicated also for A. nidulans. Composition of these complexes, however, remained obscure. In this study, we used tandem affinity purification to characterize different RpdA complexes and their composition in A. nidulans. In addition to known class 1 KDAC interactors, we identified a novel RpdA complex, which was termed RcLS2F. It contains ScrC, previously described as suppressor of the transcription factor CrzA, as well as the uncharacterized protein FscA. We show that recruitment of FscA depends on ScrC and we provide clear evidence that ΔcrzA suppression by ScrC depletion is due to a lack of transcriptional repression caused by loss of the novel RcLS2F complex. Moreover, RcLS2F is essential for sexual development and engaged in an autoregulatory feed-back loop.

Item Type:	Article
Additional Information:	Published under the Creative Commons Attribution licence (CC-BY). Cite as: Bauer I, Gross S, Merschak P, Kremser L, Karahoda B, Sarikaya Bayram Ö, Abt B, Binder U, Gsaller F, Lindner H, Bayram Ö, Brosch G and Graessle S (2020) RcLS2F – A Novel Fungal Class 1 KDAC Co-repressor Complex in Aspergillus nidulans. Funding: We acknowledge funding by the Austrian Science Fund (FWF, P24803 to StG), by the Medical University of Innsbruck (MUI Start ST201405031 to IB), and by the Science Foundation Ireland (13/CDA/2142 to ÖB). ÖSB was funded by Government of Ireland Postdoctoral Fellowship Programme (GOIPD/2014/178). Front. Microbiol. 11:43. doi: 10.3389/fmicb.2020.00043
Keywords:	RpdA; lysine deacetylase (KDAC); histone deacetylase (HDAC); Aspergillus nidulans; Ascomycota; chromatin; co-repressor complex; calcium;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	13536
Identification Number:	https://doi.org/10.3389/fmicb.2020.00043
Depositing User:	Ozgur Bayram
Date Deposited:	05 Nov 2020 17:01
Journal or Publication Title:	Frontiers in Microbiology
Publisher:	Frontiers
Refereed:	Yes
Funders:	Austrian Science Fund (FWF), Medical University of Innsbruck (MUI), Science Foundation Ireland (SFI), Government of Ireland Postdoctoral Fellowship Programme
URI:	https://www.frontiersin.org/
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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