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    Oosporein, an abundant metabolite in Beauveria caledonica, with a feedback induction mechanism and a role in insect virulence


    McNamara, Louise and Dolan, Stephen K. and Walsh, John M.D. and Stephens, John C. and Glare, Travis R. and Kavanagh, Kevin and Griffin, Christine (2019) Oosporein, an abundant metabolite in Beauveria caledonica, with a feedback induction mechanism and a role in insect virulence. Fungal Biology, 123 (8). pp. 601-610. ISSN 1878-6146

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    Abstract

    investigate the insecticidal, anti-feedant and immunomodulation effects of oosporein produced by Beauveria caledonica on the forestry pest Hylobius abietis and model insect Galleria mellonella. We report a novel feedback induction mechanism regulating oosporein production in B. caledonica; exogenous oosporein induces the expression of the oosporein cluster, leading to increased abundance of oosporein biosynthetic enzymes, as shown by label-free quantitative proteomics. Oosporein did not have an anti-feedant effect on H. abietis adults e on the contrary, insects exposed to oosporein-treated food fed more than those exposed to untreated food only. Injected oosporein did not kill insect larvae but increased susceptibility of H. abietis to a subsequent infection. Oosporein did not act as a contact toxin on H. abietis adults and G. mellonella larvae at the concentrations tested. Therefore, it appears that oosporein promotes infection rather than directly killing insects; this could be mediated both by a reduction in haemocyte numbers and by alterations to the humoral immune system. This work makes a case for future research into the potential use of B. caledonica as a biocontrol agent through combinations with oosporein or with enhanced production of oosporein

    Item Type: Article
    Additional Information: Louise Mc Namara, Stephen K. Dolan, John M.D. Walsh, John C. Stephens, Travis R. Glare, Kevin Kavanagh, Christine T. Griffin, Oosporein, an abundant metabolite in Beauveria caledonica, with a feedback induction mechanism and a role in insect virulence, Fungal Biology, Volume 123, Issue 8 (2019) Pages 601-610, ISSN 1878-6146, https://doi.org/10.1016/j.funbio.2019.01.004. Funding: This work was funded by the Department of Agriculture, Food and the Marine, as part of the MCOP project, which was funded by the Irish government under the National Development Plan 2007e2013. The quantitative mass spectrometry facilities were funded under the Science Foundation Ireland Research Infrastructure Call 2012 [grant number 12/RI/2346 (3)]. RP-HPLC facilities were funded by the higher education authority. SKD was a recipient of an Irish Research Council Embark PhD Fellowship.
    Keywords: Biocontrol; Entomopathogenic fungus; Galleria mellonella; Large pine weevil; Proteomics; Secondary metabolite;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Chemistry
    Item ID: 13591
    Identification Number: https://doi.org/10.1016/j.funbio.2019.01.004
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 19 Nov 2020 16:48
    Journal or Publication Title: Fungal Biology
    Publisher: Elsevier
    Refereed: Yes
    Funders: Department of Agriculture, Food and the Marine, Science Foundation Ireland Research Infrastructure Call 2012, Irish Research Council (IRC)
    URI:

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