Barton, Sinead and O'Dwyer, Kevin and Butler, Marion P. and Dignam, Adam and Byrne, Hugh J. and O'Neill, Luke and Hennelly, Bryan M. (2019) Improved performance of near infrared excitation Raman spectroscopy using reflective thin-film gold on glass substrates for cytology samples. Analytical Methods, 11. pp. 6023-6032. ISSN 1759-9660

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Abstract

Confocal near-infrared Raman spectroscopy has been shown to have applications in the area of clinical biology. A source wavelength in the near infrared is preferred over visible wavelengths for inspecting biological samples due to superior wavenumber resolution and reduced photodamage. However, these excitation sources have a number of drawbacks when compared to lasers in the visible wavelength region, including the requirement to use expensive highly pure crystal substrates such as Raman grade calcium fluoride as well as long acquisition times due to the lower Raman scattering efficiency. This paper investigates the use of a reflective substrate comprising a low cost 100 nm thin-film gold on glass substrate, as an alternative. Similar to recent work that used stainless steel substrates, it is demonstrated that the thin-film gold coated substrates, which are relatively inexpensive, produce cell spectra with 1.65 times the signal to noise ratio when compared with spectra obtained from calcium fluoride under identical conditions, with no apparent background signal in the fingerprint region. Two prostate cell lines are examined having been deposited on glass, calcium fluoride, and thin-film gold on glass substrates using the ThinPrep standard. Background spectra from, and cell adhesion on, these three substrates are compared. A comparison of the intensities and signal to noise ratios of the resulting spectra, and their viability for classification using principle components analysis is performed, which further demonstrates the benefit of reflective substrates.

Item Type:	Article
Additional Information:	This is the postprint version of the published article, which is available at Barton, J., O'Dwyer, K., Butler, M., Dignam,A., Byrne, H.J., O'Neill, L. & Hennelly, B.M. (2019). Improved performance of near infrared excitation Raman spectroscopy using reflective thin-film gold on glass substrates for cytology samples.Analytical Methods, 11, pp.6023-6032. doi:1039/C9AY01672d. This version is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License (https://creativecommons.org/licenses/by-nc-sa/3.0/). Funding: This research was conducted with the financial support of the Irish Research Council (IRC) under project ID GOIPG/2013/1434 and Science Foundation Ireland (SFI) under Grant Number 15/CDA/3667. I would like to thank the IRC and SFI for their support.
Keywords:	Improved performance; near infrared excitation; Raman spectroscopy; reflective thin-film; gold on glass; substrates; cytology samples;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Computer Science Faculty of Science and Engineering > Electronic Engineering Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	13593
Identification Number:	https://doi.org/10.1039/c9ay01672d
Depositing User:	Dr. Bryan Hennelly
Date Deposited:	19 Nov 2020 17:52
Journal or Publication Title:	Analytical Methods
Publisher:	Royal Society of Chemistry
Refereed:	Yes
Funders:	Irish Research Council (IRC), Science Foundation Ireland (SFI)
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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