Sheehan, Gerard and Kavanagh, Kevin (2019) Proteomic Analysis of the Responses of Candida albicans during Infection of Galleria mellonella Larvae. Journal of Fungi, 5 (7). ISSN 2309-608X

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Abstract

This study assessed the development of disseminated candidiasis within Galleria mellonella larvae and characterized the proteomic responses of Candida albicans to larval hemolymph. Infection of larvae with an inoculum of 1 × 106 yeast cells reduced larval viability 24 (53.33 ± 3.33%), 48 (33.33 ± 3.33%) and 72 (6.66 ± 3.33%) h post infection. C. albicans infection quickly disseminated from the site of inoculation and the presence of yeast and hyphal forms were found in nodules extracted from infected larvae at 6 and 24 h. A range of proteins secreted during infection of G. mellonella by C. albicans were detected in larval hemolymph and these were enriched for biological processes such as interaction with host and pathogenesis. The candicidal activity of hemolymph after immediate incubation of yeast cells resulted in a decrease in yeast cell viability (0.23 ± 0.03 × 106 yeast cells/mL), p < 0.05) as compared to control (0.99 ± 0.01 × 106 yeast cells/mL). C. albicans responded to incubation in hemolymph ex vivo by the induction of an oxidative stress response, a decrease in proteins associated with protein synthesis and an increase in glycolytic proteins. The results presented here indicate that C. albicans can overcome the fungicidal activity of hemolymph by altering protein synthesis and cellular respiration, and commence invasion and dissemination throughout the host.

Item Type:	Article
Additional Information:	© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Cite as: Sheehan, G.; Kavanagh, K. Proteomic Analysis of the Responses of Candida albicans during Infection of Galleria mellonella Larvae. J. Fungi 2019, 5, 7.
Keywords:	Candida; Galleria; infection; immunity; mini-model; in vivo screening;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	13672
Identification Number:	https://doi.org/10.3390/jof5010007
Depositing User:	Dr. Kevin Kavanagh
Date Deposited:	26 Nov 2020 15:36
Journal or Publication Title:	Journal of Fungi
Publisher:	MDPI
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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