Sheehan, Gerard and Konings, Mickey and Lim, Wilson and Fahal, Ahmed and Kavanagh, Kevin and van de Sande, Wendy W.J. (2020) Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. PLoS Neglected Tropical Diseases, 14 (4). e0008190. ISSN 1935-2727

Preview

Download (2MB) | Preview

Abstract

Mycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Characteristic of this infection is the formation of grains. However, the processes leading to grain formation are not known. In this study, we employed a proteomic approach to characterise M. mycetomatis grain formation in Galleria mellonella larvae and map the processes leading to grain formation over time. For this, at 1 day, 3 days and 7 days post-inoculation, proteins from grains and hemolymph were extracted and analysed by label-free mass spectrometry. A total of 87, 51 and 48 M. mycetomatis proteins and 713, 997, 18 G. mellonella proteins were found in grains on day 1, 3 and 7 post-inoculation respectively. M. mycetomatis proteins were mainly involved in cellular metabolic processes and numerous enzymes were encountered. G. mellonella proteins were primarily involved in the nodulation process. The proteins identified were linked to nodulation and grain formation and four steps of grain formation were identified. The results of this proteomic approach could in the future be used to design novel strategies to interfere with mycetoma grain formation and to combat this difficult to treat infection.

Item Type:	Article
Additional Information:	© 2020 Sheehan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Citation: Sheehan G, Konings M, Lim W, Fahal A, Kavanagh K, van de Sande WWJ (2020) Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. PLoS Negl Trop Dis 14(4): e0008190. https://doi.org/10.1371/journal.pntd.0008190
Keywords:	Proteomic analysis; Madurella mycetomatis grain formation; Galleria mellonella larvae; Mycetoma;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	13673
Identification Number:	https://doi.org/10.1371/journal.pntd.0008190
Depositing User:	Dr. Kevin Kavanagh
Date Deposited:	26 Nov 2020 16:18
Journal or Publication Title:	PLoS Neglected Tropical Diseases
Publisher:	Public Library of Science
Refereed:	Yes
URI:	https://journals.plos.org/
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

Repository Staff Only(login required)

Item control page

Downloads

Origin of downloads