Nugent, Jacqueline M. and Joyce, S.M. (2005) Producing Human Therapeutic Proteins in Plastids. Current Pharmaceutical Design, 11 (9). pp. 2459-2470. ISSN 1873-4286

Preview

Download (118kB) | Preview

Abstract

Plastid transformation technology is set to become a major player in the production of human therapeutic proteins. Protein expression levels that can be achieved in plant plastids are hundreds of times greater than the expression levels generally obtained via nuclear transformation. Plastids can produce human proteins that are properly folded and are biologically active. Effective protein purification strategies and strategies that can achieve inducible plastid gene expression are being developed within the system. Plastid transformation technology has been extended to edible plant species, which could minimize down-stream processing costs and raises the possibility of “edible protein therapies”. The system is limited by the fact that plastid-produced proteins are not glycosylated and that, at the moment, it can be difficult to predict protein stability within the plastid. The high level of protein expression that can be obtained in plastids could make it possible to produce high-value therapeutic proteins in plants on a scale that could be accommodated in contained glasshouse facilities and still be economically viable. Growing plastid-transformed plants under contained conditions, and coupled with the level of bio-safety conferred by maternal inheritance of plastid transgenes, would address many of the social and environmental concerns relating to plant based production of human therapeutic proteins.

Item Type:	Article
Additional Information:	Cite as: J. M. Nugent and S. M. Joyce, “ Producing Human Therapeutic Proteins in Plastids”, Current Pharmaceutical Design (2005) 11: 2459. https://doi.org/10.2174/1381612054367562
Keywords:	Transformation; plastid; plants; human; therapeutic; protein;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	13723
Identification Number:	https://doi.org/10.2174/1381612054367562
Depositing User:	Dr. Jacqueline Nugent
Date Deposited:	01 Dec 2020 15:42
Journal or Publication Title:	Current Pharmaceutical Design
Publisher:	Bentham Science Publishers
Refereed:	Yes
Funders:	Health Research Board (HRB)
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

Repository Staff Only(login required)

Item control page

Downloads

Origin of downloads