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    Producing Human Therapeutic Proteins in Plastids


    Nugent, Jacqueline M. and Joyce, S.M. (2005) Producing Human Therapeutic Proteins in Plastids. Current Pharmaceutical Design, 11 (9). pp. 2459-2470. ISSN 1873-4286

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    Abstract

    Plastid transformation technology is set to become a major player in the production of human therapeutic proteins. Protein expression levels that can be achieved in plant plastids are hundreds of times greater than the expression levels generally obtained via nuclear transformation. Plastids can produce human proteins that are properly folded and are biologically active. Effective protein purification strategies and strategies that can achieve inducible plastid gene expression are being developed within the system. Plastid transformation technology has been extended to edible plant species, which could minimize down-stream processing costs and raises the possibility of “edible protein therapies”. The system is limited by the fact that plastid-produced proteins are not glycosylated and that, at the moment, it can be difficult to predict protein stability within the plastid. The high level of protein expression that can be obtained in plastids could make it possible to produce high-value therapeutic proteins in plants on a scale that could be accommodated in contained glasshouse facilities and still be economically viable. Growing plastid-transformed plants under contained conditions, and coupled with the level of bio-safety conferred by maternal inheritance of plastid transgenes, would address many of the social and environmental concerns relating to plant based production of human therapeutic proteins.

    Item Type: Article
    Additional Information: Cite as: J. M. Nugent and S. M. Joyce, “ Producing Human Therapeutic Proteins in Plastids”, Current Pharmaceutical Design (2005) 11: 2459. https://doi.org/10.2174/1381612054367562
    Keywords: Transformation; plastid; plants; human; therapeutic; protein;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 13723
    Identification Number: https://doi.org/10.2174/1381612054367562
    Depositing User: Dr. Jacqueline Nugent
    Date Deposited: 01 Dec 2020 15:42
    Journal or Publication Title: Current Pharmaceutical Design
    Publisher: Bentham Science Publishers
    Refereed: Yes
    Funders: Health Research Board (HRB)
    URI:

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