MURAL - Maynooth University Research Archive Library



    The lethal and sub-lethal consequences of entomopathogenic nematode infestation and exposure for adult pine weevils, Hylobius abietis (Coleoptera: Curculionidae)


    Girling, R.D. and Ennis, D. and Dillon, A.B. and Griffin, Christine (2010) The lethal and sub-lethal consequences of entomopathogenic nematode infestation and exposure for adult pine weevils, Hylobius abietis (Coleoptera: Curculionidae). Journal of Invertebrate Pathology, 104 (3). pp. 195-202. ISSN 0022-2011

    [img] Download (1MB)


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    Entomopathogenic nematodes (EPN) frequently kill their host within 1–2 days, and interest in EPN focuses mainly on their lethality. However, insects may take longer to die, or may fail to die despite being infected, but little is known about the effects of EPN infection on insects, other than death. Here we investigate both lethal and sub-lethal effects of infection by two EPN species, Steinernema carpocapsae and Heterorhabditis downesi, on adults of the large pine weevil, Hylobius abietis. Following 12 h nematode–weevil contact in peat, S. carpocapsae killed a significantly higher proportion of weevils (87–93%) than H. downesi (43–57%) at all concentrations tested. Less than 10% of weevils were dead within 2 days, and weevils continued to die for up to 10 days after exposure (LT50 of 3 days or more). In a separate experiment, live weevils dissected 6 days after a 24 h exposure to nematodes on filter paper harbored encapsulated and dead nematodes, showing that weevils could defend themselves against infection. Some live weevils also harbored live nematodes 6 days after they had been removed from the nematode infested medium. Feeding by weevils was not affected by infection with, or exposure to, either species of EPN. We discuss these results in relation to the use of EPN in biological control against H. abietis.

    Item Type: Article
    Additional Information: The definitive version of this article is available at doi:10.1016/j.jip.2010.04.003
    Keywords: Hylobius abietis; Steinernema carpocapsae; Heterorhabditis downesi; Feeding behavior; Biological control; LT50; LT90;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 4127
    Depositing User: Dr. Christine Griffin
    Date Deposited: 30 Jan 2013 14:28
    Journal or Publication Title: Journal of Invertebrate Pathology
    Publisher: Elsevier
    Refereed: Yes
    URI:

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year