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    Effect of nutrient deprivation on the susceptibility of Galleria mellonella larvae to infection


    Banville, Nessa and Browne, Niall and Kavanagh, Kevin (2012) Effect of nutrient deprivation on the susceptibility of Galleria mellonella larvae to infection. Virulence, 3 (6). pp. 497-503. ISSN 2150-5594

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    Abstract

    Larvae of Galleria mellonella are widely used to study the virulence of microbial pathogens and for assessing the potency of antimicrobial agents. This work examined the effect of nutritional deprivation on the ability of larvae to withstand infection in order to establish standardized conditions for the treatment of larvae for in vivo testing. Larvae deprived of food for seven days demonstrated an increased susceptibility to infection by the yeast Candida albicans. These larvae displayed a lower density of hemocytes compared with controls but hemocytes from starved and control larvae demonstrated the same ability to kill yeast cells. Hemolymph from starved larvae demonstrated reduced expression of a range of antimicrobial peptides (e.g., lipocalin) and immune proteins (e.g., apolipophorin and arylphorin). Deprivation of G. mellonella larvae of food leads to a reduction in the cellular and immune responses and an increased susceptibility to infection. Researchers utilizing these larvae should ensure adequate food is provided to larvae in order to allow valid comparisons to be made between results from different laboratories.

    Item Type: Article
    Keywords: Candida; Galleria; in vivo testing; microbial pathogens; mini-model;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 4189
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 05 Feb 2013 11:15
    Journal or Publication Title: Virulence
    Publisher: Landes Bioscience
    Refereed: Yes
    URI:

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