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    Characterisation of expression patterns and functional role of Cactin in early zebrafish development


    Atzei, Paola and Yang, Fan and Collery, Ross and Kennedy, Breandan N. and Moynagh, Paul N. (2010) Characterisation of expression patterns and functional role of Cactin in early zebrafish development. Gene Expression Patterns, 10 (4/5). pp. 199-206. ISSN 1567-133X

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    Abstract

    The immune system of teleost zebrafish (Danio rerio) shows high similarity to mammalian counterparts sharing many innate immune components including Toll-Like Receptors (TLRs), cytokines, chemokines and complement molecules. As in mammals, zebrafish also contains the transcription factor NF-jB that plays dualist roles in innate immunity and early development. Indeed NF-jB members are expressed in different temporal patterns during the early stages of zebrafish embryogenesis indicating that each molecule is involved in specific developmental events. In the present study we employ zebrafish as a model to characterise the expression pattern and role of a novel NF-jB regulator, termed Cactin, in early development. Cactin was first characterised in Drosophila as a new member of the Rel pathway that could affect the generation of dorsal–ventral polarity. To explore the potential developmental role of Cactin in zebrafish, we initially investigated its expression pattern and functional role during early embryonic developmental stages. We detect Cactin expression at all stages of early development and knockdown of Cactin by specific morpholino antisense oligonucleotides causes developmental abnormalities manifested by an overall dysmorphic cellular organisation. These results indicate that Cactin has been highly conserved during evolution and plays a key role in early embryonic development.

    Item Type: Article
    Additional Information: The definitive version of this article is available at doi:10.1016/j.gep.2010.03.003
    Keywords: Toll-like receptors; NF-jB; Cactin; Early development; In situ hybridisation; Morpholino;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Research Institutes > Institute of Immunology
    Item ID: 4283
    Depositing User: Professor Paul Moynagh
    Date Deposited: 03 Apr 2013 16:09
    Journal or Publication Title: Gene Expression Patterns
    Publisher: Elsevier
    Refereed: Yes
    URI:

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