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    The Culturable Soil Antibiotic Resistome: A Community of Multi-Drug Resistant Bacteria


    Walsh, Fiona and Duffy, Brion (2013) The Culturable Soil Antibiotic Resistome: A Community of Multi-Drug Resistant Bacteria. PLoS ONE. ISSN 1932-6203

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    Official URL: http://www.plosone.org/article/info%3Adoi%2F10.137...


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    Abstract

    Understanding the soil bacterial resistome is essential to understanding the evolution and development of antibiotic resistance, and its spread between species and biomes. We have identified and characterized multi-drug resistance (MDR) mechanisms in the culturable soil antibiotic resistome and linked the resistance profiles to bacterial species. We isolated 412 antibiotic resistant bacteria from agricultural, urban and pristine soils. All isolates were multi-drug resistant, of which greater than 80% were resistant to 16–23 antibiotics, comprising almost all classes of antibiotic. The mobile resistance genes investigated, (ESBL, bla NDM-1 , and plasmid mediated quinolone resistance (PMQR) resistance genes) were not responsible for the respective resistance phenotypes nor were they present in the extracted soil DNA. Efflux was demonstrated to play an important role in MDR and many resistance phenotypes. Clinically relevant Burkholderia species are intrinsically resistant to ciprofloxacin but the soil Burkholderia species were not intrinsically resistant to ciprofloxacin. Using a phenotypic enzyme assay we identified the antibiotic specific inactivation of trimethoprim in 21 bacteria from different soils. The results of this study identified the importance of the efflux mechanism in the soil resistome and variations between the intrinsic resistance profiles of clinical and soil bacteria of the same family

    Item Type: Article
    Keywords: Culturable Soil Antibiotic Resistome; Community of Multi-Drug Resistant Bacteria;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 4813
    Identification Number: https://doi.org/10.1371/journal.pone.0065567
    Depositing User: Fiona Walsh
    Date Deposited: 11 Mar 2014 15:21
    Journal or Publication Title: PLoS ONE
    Publisher: Public Library of Science
    Refereed: Yes
    URI:

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