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    Proteomics as the final step in the functional metagenomics study of antimicrobial resistance


    Fouhy, Fiona and Stanton, Catherine and Cotter, Paul D. and Hill, Colin and Walsh, Fiona (2015) Proteomics as the final step in the functional metagenomics study of antimicrobial resistance. Frontiers in Microbiology, 6 (172). pp. 1-6. ISSN 1664-302X

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    Abstract

    The majority of clinically applied antimicrobial agents are derived from natural products generated by soil microorganisms and therefore resistance is likely to be ubiquitous in such environments. This is supported by the fact that numerous clinically important resistance mechanisms are encoded within the genomes of such bacteria. Advances in genomic sequencing have enabled the in silico identification of putative resistance genes present in these microorganisms. However, it is not sufficient to rely on the identification of putative resistance genes, we must also determine if the resultant proteins confer a resistant phenotype. This will require an analysis pipeline that extends from the extraction of environmental DNA, to the identification and analysis of potential resistance genes and their resultant proteins and phenotypes. This review focuses on the application of functional metagenomics and proteomics to study antimicrobial resistance in diverse environments.

    Item Type: Article
    Additional Information: Copyright © 2015 Fouhy, Stanton, Cotter, Hill and Walsh. This is an open-access article distributed under the terms of the Creative Commons Attribution License(CCBY). The use, distribution or reproduction in other forums is permitted, pro vided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
    Keywords: functional metagenomics; antibiotic resistance; proteins; microbiology; novel mechanisms;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6184
    Identification Number: https://doi.org/10.3389/fmicb.2015.00172
    Depositing User: Fiona Walsh
    Date Deposited: 10 Jun 2015 14:44
    Journal or Publication Title: Frontiers in Microbiology
    Publisher: Frontiers
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI)
    URI:

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