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    Systematic Global Analysis of Genes Encoding Protein Phosphatases in Aspergillus fumigatus


    Winkelstroter, Lizziane K. and Dolan, Stephen K. and Fernanda dos Reis, Thaila and Pedro Bom, Vinicius Leite and Alves de Castro, Patricia and Hagiwara, Daisuke and Alowni, Raneem and Jones, Gary W. and Doyle, Sean and Brown, Neil Andrew and Goldman, Gustavo H. (2015) Systematic Global Analysis of Genes Encoding Protein Phosphatases in Aspergillus fumigatus. G3 : Genes, Genomes, Genetics, 5. pp. 1525-1539. ISSN 2160-1836

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    Abstract

    Aspergillus fumigatus is a fungal pathogen that causes several invasive and noninvasive diseases named aspergillosis. This disease is generally regarded as multifactorial, considering that several pathogenicity determinants are present during the establishment of this illness. It is necessary to obtain an increased knowledge of how, and which, A. fumigatus signal transduction pathways are engaged in the regulation of these processes. Protein phosphatases are essential to several signal transduction pathways. We identified 32 phosphatase catalytic subunit-encoding genes in A. fumigatus, of which we were able to construct 24 viable deletion mutants. The role of nine phosphatase mutants in the HOG (high osmolarity glycerol response) pathway was evaluated by measuring phosphorylation of the p38 MAPK (SakA) and expression of osmo-dependent genes. We were also able to identify 11 phosphatases involved in iron assimilation, six that are related to gliotoxin resistance, and three implicated in gliotoxin production. These results present the creation of a fundamental resource for the study of signaling in A. fumigatus and its implications in the regulation of pathogenicity determinants and virulence in this important pathogen.

    Item Type: Article
    Additional Information: This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/ licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Supporting information is available online at www.g3journal.org/lookup/suppl/ doi:10.1534/g3.115.016766/-/DC1
    Keywords: Aspergillus fumigatus; HOG; phosphatases; gliotoxin; iron metabolism; protein; phosphatases;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6337
    Identification Number: https://doi.org/10.1534/g3.115.016766
    Depositing User: Dr. Sean Doyle
    Date Deposited: 07 Sep 2015 15:35
    Journal or Publication Title: G3 : Genes, Genomes, Genetics
    Publisher: Genetics Society of America
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Higher Education Authority (HEA)
    URI:

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