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    Assessment of in vivo antimicrobial activity of the carbene silver(I) acetate derivative SBC3 using Galleria mellonella larvae


    Browne, Niall and Hackenberg, Frauke and Streciwilk, Wojciech and Tacke, Matthias and Kavanagh, Kevin (2014) Assessment of in vivo antimicrobial activity of the carbene silver(I) acetate derivative SBC3 using Galleria mellonella larvae. Biometals, 27 (4). pp. 745-752. ISSN 0966-0844

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    Abstract

    The antimicrobial drug candidate 1,3- dibenyl-4,5-diphenyl-imidazol-2-ylidene silver(I) acetate (SBC3) was evaluated for its ability to function in vivo using larvae of Galleria mellonella. A SBC3 concentration of 25 lg/ml inhibited the growth of Staphylococcus aureus by 71.2 % and Candida albicans by 86.2 % in vitro. Larvae inoculated with 20 ll of SBC3 solution showed no ill effects up to a concentration of 250 lg/ml but administration of 500 lg/ml resulted in a 40 % reduction in larval survival and administration of a dose of 1,000 lg/ml resulted in total larval death at 24 h. Larvae inoculated with S. aureus or C. albicans and subsequently administered SBC3 showed increased survival. Administration of SBC3 to larvae did not boost the insect immune response as indicated by lack of an increase in the density of circulating haemocytes (immune cells). The abundance of a number of proteins involved in the insect immune response was reduced in larvae that received 20 ll SBC3 solution of 100 lg/ml. This is the first demonstration of the in vivo activity of SBC3 against S. aureus and C. albicans and demonstrates that SBC3 does not stimulate a non-specific immune response in larvae.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1007/s10534-014-9766-z
    Keywords: Antimicrobial drug; Candida albicans; Carbene-silver complex; Galleria mellonella; In vivo toxicity; Staphylococcus aureus;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6896
    Identification Number: https://doi.org/10.1007/s10534-014-9766-z
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 20 Jan 2016 13:19
    Journal or Publication Title: Biometals
    Publisher: Springer Verlag
    Refereed: Yes
    URI:

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