Carberry, Steven and Zweyer, Margit and Swandulla, Dieter and Ohlendieck, Kay
(2013)
Application of Fluorescence Two-Dimensional Difference In-Gel
Electrophoresis as a Proteomic Biomarker Discovery Tool in
Muscular Dystrophy Research.
Biology, 2.
pp. 1438-1464.
ISSN 2079-7737
Abstract
In this article, we illustrate the application of difference in-gel electrophoresis
for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used
as a tissue model of dystrophinopathy. Two-dimensional gel electrophoresis is a widely
employed protein separation method in proteomic investigations. Although two-dimensional
gels usually underestimate the cellular presence of very high molecular mass proteins,
integral membrane proteins and low copy number proteins, this method is extremely
powerful in the comprehensive analysis of contractile proteins, metabolic enzymes,
structural proteins and molecular chaperones. This gives rise to two-dimensional gel
electrophoretic separation as the method of choice for studying contractile tissues in health
and disease. For comparative studies, fluorescence difference in-gel electrophoresis has
been shown to provide an excellent biomarker discovery tool. Since aged diaphragm fibres
from the mdx mouse model of Duchenne muscular dystrophy closely resemble the human
pathology, we have carried out a mass spectrometry-based comparison of the naturally
aged diaphragm versus the senescent dystrophic diaphragm. The proteomic comparison of
wild type versus mdx diaphragm resulted in the identification of 84 altered protein species.
Novel molecular insights into dystrophic changes suggest increased cellular stress,
impaired calcium buffering, cytostructural alterations and disturbances of mitochondrial
metabolism in dystrophin-deficient muscle tissue.*
| Item Type: |
Article
|
| Additional Information: |
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited: Carberry, S.; Zweyer, M.; Swandulla, D.; Ohlendieck, K. Application of Fluorescence Two-Dimensional Difference In-Gel Electrophoresis as a Proteomic Biomarker Discovery Tool in Muscular Dystrophy Research. Biology 2013, 2, 1438-1464. |
| Keywords: |
cvHsp; diaphragm; DIGE; Duchenne muscular dystrophy; dystrophinopathy;
gel electrophoresis; HspB7; mdx; mouse model; parvalbumin; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6900 |
| Identification Number: |
https://doi.org/10.3390/biology2041438 |
| Depositing User: |
Prof. Kay Ohlendieck
|
| Date Deposited: |
21 Jan 2016 14:53 |
| Journal or Publication Title: |
Biology |
| Publisher: |
MDPI |
| Refereed: |
Yes |
| Funders: |
Muscular Dystrophy Ireland, Duchenne Ireland, Hume Scholarship, Health Research Board (HRB), Higher Education Authority (HEA) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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