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    The use of LC-MS to identify differentially expressed proteins in docetaxel-resistant prostate cancer cell lines


    O'Connell, Kathleen and Prencipe, Maria and O'Neill, Amanda and Corcoran, Claire and Rani, Sweta and Henry, Michael and Dowling, Paul and Meleady, Paula and O'Driscoll, Lorraine and Watson, William and O'Connor, Robert (2012) The use of LC-MS to identify differentially expressed proteins in docetaxel-resistant prostate cancer cell lines. Proteomics, 12 (13). pp. 2115-2126. ISSN 1615-9853

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    Abstract

    Docetaxel is a taxane-derived chemotherapy drug that has been approved for treatment of prostate cancer. While docetaxel is frequently used as a treatment for hormone-refractory prostate cancer, a subset of patients either do not respond to this treatment or those that do respond eventually become resistant to the drug over time. Resistance to docetaxel is complex and multi-factoral and further understanding of the cellular biochemistry underlying resistance is vital to improve treatment efficacy. To identify proteins altered in the resistant phenotype, three parental cell lines DU145, 22RV1 and PC-3, as well as their docetaxel resistant sub-lines, were subjected to quantitative label-free LC-MS proteomic profiling. A total of 189 significant (p < 0.05) protein abundance changes were identified in the DU145 resistant sub-lines, 254 in the 22RV1 sub-lines, and 51 and 72 in the 8 and 12 nM resistant PC-3 sub-lines, respectively. From these, 29 proteins demonstrated a significant (p < 0.05) fold change across two or more resistant variants. These included proteins indicative of an epithelial-to-mesenchemyl transition as well as altered heat shock response elements.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1002/pmic.201100489
    Keywords: Cancer; Cell biology; Docetaxel; Prostate; Resistant;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6926
    Identification Number: https://doi.org/10.1002/pmic.201100489
    Depositing User: Paul Dowling
    Date Deposited: 26 Jan 2016 17:32
    Journal or Publication Title: Proteomics
    Publisher: Wiley
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI)
    URI:

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