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    Olfactomedin III expression contributes to anoikis-resistance in clonal variants of a human lung squamous carcinoma cell line


    Keenan, Joanne and Joyce, Helena and Aherne, Sinead and O'Dea, Shirley and Doolan, Padraig and Lynch, Vincent and Clynes, Martin (2012) Olfactomedin III expression contributes to anoikis-resistance in clonal variants of a human lung squamous carcinoma cell line. Experimental Cell Research, 318 (5). pp. 593-602. ISSN 0014-4827

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    Abstract

    Three clonal subpopulations of DLKP, a poorly differentiated squamous lung carcinoma cell line, display striking differences in ability to survive in suspension (anoikis resistance). DLKP-SQ is anoikis resistant (7.5% anoikis at 24 h). In contrast, DLKP-M and DLKP-I are sensitive to anoikis (49.2% and 42.6% respectively). DLKP-I shows increased apoptosis consistently over all time points tested while DLKP-M appear to slow down metabolically and perhaps delays onset of anoikis by undergoing autophagy. Expression microarray analysis identified pronounced differential expression of Olfactomedin 3 (OLFM3) between the clones. High expression of OLFM3 was confirmed at the RNA level by qRT-PCR in DLKP-SQ and at the protein level by Western blotting (within the cell and secreted). Little or no OLFM3 was detected in the other two clones (DLKPM and DLKP-I). Following siRNA knockdown of OLFM3 in DLKP-SQ, anoikis was increased 2.8- fold to 21% which was intermediate between the anoikis levels in DLKP-SQ and DLKP-M or DLKP-I. This knockdown correlated with increased apoptosis in suspension but not in attached culture conditions. Addition of recombinant OLFM3 reduced anoikis in DLKP-I. This is the first instance of OLFM3 being linked with anoikis resistance in a human cancer cell line.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1016/j.yexcr.2012.01.012
    Keywords: Olfactomedin 3; Anoikis; Lung cancer;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Research Institutes > Institute of Immunology
    Item ID: 6930
    Identification Number: https://doi.org/10.1016/j.yexcr.2012.01.012
    Depositing User: Dr. Shirley O'Dea
    Date Deposited: 29 Jan 2016 15:27
    Journal or Publication Title: Experimental Cell Research
    Publisher: Elsevier
    Refereed: Yes
    Funders: Higher Education Authority (HEA), Science Foundation Ireland (SFI)
    URI:

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