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    Absence of p53 in Clara cells favours multinucleation and loss of cell cycle arrest


    Armit, Christopher J. and O'Dea, Shirley and Clarke, Alan R. and Harrison, David J. (2002) Absence of p53 in Clara cells favours multinucleation and loss of cell cycle arrest. BMC Cell Biology, 3 (27). ISSN 1471-2121

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    Official URL: http://www.biomedcentral.com/1471-2121/3/27


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    Abstract

    Background The p53 oncosuppressor protein is a critical mediator of the response to injury in mammalian cells and is mutationally inactivated in the majority of lung malignancies. In this analysis, the effects of p53-deficiency were investigated in short-term primary cultures of murine bronchiolar Clara cells. Clara cells, isolated from gene-targeted p53-deficient mice, were compared to cells derived from wild type littermates. Results p53 null cultures displayed abnormal morphology; specifically, a high incidence of multinucleation, which increased with time in culture. Multinucleated cells were proficient in S phase DNA synthesis, as determined by BrdU incorporation. However, multinucleation did not reflect altered rates of S phase synthesis, which were similar between wild type and p53-/- cultures. Nucleation defects in p53-/- Clara cells associated with increased centrosome number, as determined by confocal microscopy of pericentrin-stained cultures, and may highlight a novel role of p53 in preserving genomic integrity in lung epithelial cells. Effects of p53-deficiency were also studied following exposure to DNA damage. A p53-dependent reduction in the BrdU index was observed in Clara cells following ionizing radiation. The reduction in BrdU index in wild type cells displayed serum-dependency, and occurred only in the absence of serum. Taken together, these findings demonstrate that in murine primary Clara cell culture, cell cycle arrest is a p53-mediated response to DNA damage, and that extracellular factors, such as serum, influence this response. Conclusion These findings highlight functions of wild type p53 protein in bipolar spindle formation, centrosome regulation, and growth control in bronchiolar Clara cells.

    Item Type: Article
    Additional Information: © 2002 Armit et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
    Keywords: p53 oncosuppressor protein; bronchiolar Clara cells; multinucleation; loss of cell cycle arrest;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Research Institutes > Institute of Immunology
    Item ID: 6935
    Identification Number: https://doi.org/10.1186/1471-2121-3-27
    Depositing User: Dr. Shirley O'Dea
    Date Deposited: 01 Feb 2016 09:21
    Journal or Publication Title: BMC Cell Biology
    Publisher: BioMed Central
    Refereed: Yes
    Funders: Norman Salvesen Emphysema Trust
    URI:

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