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    Highly selective and stable microdisc biosensors for l-glutamate monitoring


    Govindarajan, Sridhar and McNeil, Calum J. and Lowry, John P. and McMahon, Colm P. and O'Neill, Robert D. (2013) Highly selective and stable microdisc biosensors for l-glutamate monitoring. Sensors and Actuators B, 178. pp. 606-614. ISSN 0925-4005

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    Abstract

    Glutamate mediates most of the excitatory synaptic transmission in the brain, and its abnormal regulation is considered a key factor underlying the appearance and progression of many neurodegenerative and psychiatric diseases. In this work, a microdisc-based amperometric biosensor for glutamate detection with highly enhanced selectivity and good stability is proposed. The biosensor utilizes the enzyme glutamate oxidase which was dip-coated onto 125 um diameter platinum discs. To improve selectivity, phosphatidylethanolamine was pre-coated prior to enzyme deposition, and electropolymerization of o-phenylenediamine was performed to entrap the enzyme within a polymer matrix. A variety of coating configurations were tested in order to optimize biosensor performance. For stability measurements, biosensors were biased continuously and calibration curves calculated each day for a period of 5–6 days. The optimized biosensors exhibited very high sensitivity (71 ± 1 mA M−1 cm−2), low detection limit of ∼2.5 uM glutamate, selectivity (over 87% against ascorbic acid), very good temporal stability during continuous use, and a response time of <5 s. These biosensors are therefore good candidates for further development as devices for continuous monitoring during traumatic brain injury or neurosurgery.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1016/j.snb.2012.12.077
    Keywords: Glutamate; Biosensor; o-Phenylenediamine; Nafion; Selectivity; Stability;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 7011
    Identification Number: https://doi.org/10.1016/j.snb.2012.12.077
    Depositing User: John Lowry
    Date Deposited: 09 Mar 2016 10:29
    Journal or Publication Title: Sensors and Actuators B
    Publisher: Elsevier
    Refereed: Yes
    URI:

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