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    Antigenic Sequences of Poliovirus Recognized by T Cells: Serotype-Specific Epitopes on VP1 and VP3 and Cross-Reactive Epitopes on VP4 Defined by Using CD4+ T-Cell Clones


    Mahon, Bernard P. and Katrak, Kersi and Mills, Kingston H.G. (1992) Antigenic Sequences of Poliovirus Recognized by T Cells: Serotype-Specific Epitopes on VP1 and VP3 and Cross-Reactive Epitopes on VP4 Defined by Using CD4+ T-Cell Clones. Journal of Virology, 66 (12). pp. 7012-7020. ISSN 0022-538X

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    Abstract

    A panel of poliovirus-specific murine CD4+ T-cell clones has been established from both BALB/c (H-2d) and CBA (H-2k) mice immunized with Sabin vaccine strains of poliovirus serotype 1, 2, or 3. T-cell clones were found to be either serotype specific or cross-reactive between two or all three serotypes. Specificity analysis against purified poliovirus proteins demonstrated that T-cell clones recognized determinants on the surface capsid proteins VP1, VP2, and VP3 and the internal capsid protein VP4. Panels of overlapping synthetic peptides were used to identify eight distinct T-cell epitopes. One type 3-specific T-cell clone recognized an epitope within amino acids 257 and 264 of VP1. Three T-cell epitopes corresponding to residues 14 to 28, 189 to 203, and 196 to 210 were identified on VP3 of poliovirus type 2. The remaining four T-cell epitopes were mapped to an immunodominant region of VP4, encompassed within residues 6 and 35 and recognized by both H-2Z and H-2k mice. The epitopes on VP4 were conserved between serotypes, and this may account for the predominantly cross-reactive poliovirus-specific T-cell response observed with polyclonal T-cell populations. In contrast, T-cell clones that recognize epitopes on VP1 or VP3 were largely serotype specific; single or multiple amino acid substitutions were found to be critical for T-cell recognition.

    Item Type: Article
    Keywords: Antigenic Sequences; Poliovirus; T Cells: Serotype-Specific Epitopes; VP1; VP3; Cross-Reactive Epitopes; VP4; CD4+; T-Cell Clones;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7144
    Depositing User: Bernard Mahon
    Date Deposited: 01 Jul 2016 09:06
    Journal or Publication Title: Journal of Virology
    Publisher: American Society for Microbiology
    Refereed: Yes
    URI:

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