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    Atypical Disease after Bordetella pertussis Respiratory Infection of Mice with Targeted Disruptions of Interferon-y Receptor or Immunoglobulin µ Chain Genes


    Mahon, Bernard P. and Sheahan, Brian J. and Griffin, Fiona and Murphy, Geraldine (1997) Atypical Disease after Bordetella pertussis Respiratory Infection of Mice with Targeted Disruptions of Interferon-y Receptor or Immunoglobulin µ Chain Genes. Journal of Experimental Medicine, 186 (11). pp. 1843-1851. ISSN 0022-1007

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    Abstract

    Using a murine respiratory challenge model we have previously demonstrated a role for Th1 cells in natural immunity against Bordetella pertussis, but could not rule out a role for antibody. Here we have demonstrated that B. pertussis respiratory infection of mice with targeted disruptions of the genes for the IFNy receptor resulted in an atypical disseminated disease which was lethal in a proportion of animals, and was characterized by pyogranulomatous inflammation and postnecrotic scarring in the livers, mesenteric lymph nodes and kidneys. Viable virulent bacteria were detected in the blood and livers of diseased animals. An examination of the course of infection in the lung of IFNy receptor–deficient, IL-4–deficient and wild-type mice demonstrated that lack of functional IFNy or IL-4, cytokines that are considered to play major roles in regulating the development of Th1 and Th2 cells, respectively, did not affect the kinetics of bacterial elimination from the lung. In contrast, B cell–deficient mice developed a persistent infection and failed to clear the bacteria after aerosol inoculation. These findings demonstrate an absolute requirement for B cells or their products in the resolution of a primary infection with B. pertussis, but also define a critical role for IFNy in containing bacteria to the mucosal site of infection.

    Item Type: Article
    Keywords: Atypical Disease; Bordetella pertussis; Respiratory Infection; Mice; Targeted Disruptions; Interferon-y Receptor; Immunoglobulin µ Chain Genes;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7146
    Depositing User: Bernard Mahon
    Date Deposited: 01 Jul 2016 09:05
    Journal or Publication Title: Journal of Experimental Medicine
    Publisher: Rockefeller University Press
    Refereed: Yes
    Funders: Wellcome Trust
    URI:

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