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    Cellular and humoral immune responses to poliovirus in mice: a role for helper T cells in heterotypic immunity to poliovirus


    Katrak, K. and Mahon, Bernard P. and Minor, P.D. and Mills, K.H.G. (1991) Cellular and humoral immune responses to poliovirus in mice: a role for helper T cells in heterotypic immunity to poliovirus. Journal of General Virology, 72. pp. 1093-1098. ISSN 0022-1317

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    Abstract

    Immunization of BALB/c mice with a single dose of the Sabin type 1, type 2 or type 3 poliovirus vaccine strains stimulated cross-reactive T helper cell responses detected by both in vitro proliferation and interleukin (IL)-2/IL-4 production. Although the polyclonal T cell responses were cross-reactive, the results also suggest that a proportion of the T cells were directed against serotype-specific determinants. In contrast, neutralizing antibodies, assayed in the serum from the same animals, were predominantly serotype-specific and only reached significant titres after secondary immunization. A comparison of the immunogenicity of poliovirus administered subcutaneously in Freund's complete adjuvant or intraperitoneally as an alum precipitate or without adjuvant, showed that optimum responses were obtained by immunization with virus in the presence of alum. An examination of the effect of heterotypic priming showed that immunization with type 2 virus primed for a secondary antibody response to each of the three serotypes, whereas priming with type 1 or type 3 viruses could only generate a secondary antibody response to the homologous virus or to type 2 virus.

    Item Type: Article
    Keywords: Cellular; humoral; immune responses; poliovirus; mice; helper T cells; heterotypic immunity;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7147
    Identification Number: https://doi.org/10.1099/0022-1317-72-5-1093
    Depositing User: Bernard Mahon
    Date Deposited: 01 Jul 2016 09:04
    Journal or Publication Title: Journal of General Virology
    Publisher: Microbiology Society
    Refereed: Yes
    URI:

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