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    Preparation and Characterization of Monoclonal Antibodies Directed Against Antigenic Determinants of Recombinant Human Tumour Necrosis Factor (rTNF)


    Meager, Anthony and Parti, Sharan and Leung, Helen and Peil, Elizabeth and Mahon, Bernard P. (1987) Preparation and Characterization of Monoclonal Antibodies Directed Against Antigenic Determinants of Recombinant Human Tumour Necrosis Factor (rTNF). Hybridoma, 6 (3). pp. 305-311. ISSN 1554-0014

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    Abstract

    A large number of monoclonal antibodies (McAb) binding to antigenic determinants of human tumour necrosis factor (TNF) were prepared from two fusions of mouse myeloma NSO cells with spleen cells from Balb/c mice immunized with highly purified recombinant (r)TNF. Several of these McAbs were highly neutralizing with respect to the biological activity (cytotoxicity) of TNF manifested in L-929 C1.10 cells. Antibody competition experiments suggested the presence of at least two antigenic determinants on the rTNF molecule through which binding of McAb effects neutralization of biological activity. Some of these McAbs were shown to be suitable for the development of immuoassays to quantify rTNF.

    Item Type: Article
    Keywords: Preparation; Characterization; Monoclonal Antibodies; Antigenic Determinants; Recombinant Human Tumour Necrosis Factor; rTNF;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7160
    Depositing User: Bernard Mahon
    Date Deposited: 06 Jul 2016 09:59
    Journal or Publication Title: Hybridoma
    Publisher: Mary Ann Lieber!, Inc.
    Refereed: Yes
    URI:

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