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    HIV p24-Specific Helper T Cell Clones From Immunised Primates Recognize Highly Conserved Regions of HIV-l


    Mills, Kingston H.G. and Kitchin, Peter A. and Mahon, Bernard P. and Barnard, Amanda L. and Adams, Sally E. and Kingsman, Susan M. and Kingsman, Alan J. (1990) HIV p24-Specific Helper T Cell Clones From Immunised Primates Recognize Highly Conserved Regions of HIV-l. Journal of Immunology, 144 (5). pp. 1677-1683. ISSN 0022-1767

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    Abstract

    We have investigated Th cell recognition of the HIV core protein p24 by using CD4+ T cell clones derived from cynomolgus macaques immunized with hybrid HIV p24:Ty virus-like particles (VLP). T cell lines from two immunized animals responded to p24:Ty-VLP, control Ty-VLP, purified p24, and whole inactivated HIV, indicating the presence of T cells specific for p24 as well as the Ty carrier protein. The HIV determinants recognized by the T cell lines were identified by using a series of overlapping peptides synthesized according to the sequence of p24. Both T cell lines recognized peptide 11 (amino acids 235-249) and peptide 14 (amino acids 265- 279). In addition, one T cell line also responded to peptide 9 (amino acids 215-229). Definitive identification of two T cell epitopes on p24 was confirmed at the clonal level: from a total of four T cell clones generated from one of the T cell lines, two respond specifically to peptide 11 and two to peptide 14. The T cell clones were CD4' and MHC class 11-restricted and secreted IL-2 in response to stimulation with purified p24, inactivated HIV or a single synthetic peptide. The specificityof the Th clones for variant peptides demonstrated cross-reactivity with two simian immunodeficiency virus isolates, but only limited responses to HIV-2 sequences. However, the Th cell epitopes identified on p24 are highly conserved between 12 HIV-1 isolates and were recognized by both of the immunized primates. These sequences may therefore be useful for priming a broadly reactive immune response to HIV-1.

    Item Type: Article
    Keywords: HIV p24-Specific; Helper; T Cell; Clones; Immunized Primates; HIV-l; Th cell recognition; HIV core protein p24;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7165
    Depositing User: Bernard Mahon
    Date Deposited: 06 Jul 2016 15:31
    Journal or Publication Title: Journal of Immunology
    Publisher: American Association of Immunologists
    Refereed: Yes
    URI:

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