MURAL - Maynooth University Research Archive Library



    Phylogenetics of Papaver and Related Genera Based on DNA Sequences from ITS Nuclear Ribosomal DNA and Plastid trnL Intron and trnL–F Intergenic Spacers


    Carolan, James C. and Hook, Ingrid L.I. and Chanse, Mark W. and Kadereit, Joachim W. and Hodkinson, Trevor R. (2006) Phylogenetics of Papaver and Related Genera Based on DNA Sequences from ITS Nuclear Ribosomal DNA and Plastid trnL Intron and trnL–F Intergenic Spacers. Annals of Botany, 98 (1). pp. 141-155. ISSN 0305-7364

    [img]
    Preview
    Download (179kB) | Preview


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    _ Background and Aims Representatives from Papaver, Roemeria, Stylomecon and Meconopsis were studied to elucidate phylogenetic relationships between Papaver and these closely allied genera. _ Methods Two molecular data sets were used individually and combined and included sequences from the internally transcribed spacer region (ITS) of 18S–26S nuclear ribosomal DNA and the trnL intron and the trnL–trnF intergenic spacer region of plastid DNA. _ Key Results Parsimony analysis demonstrated that the genus is not monophyletic unless the closely related Roemeria, Stylomecon and Meconopsis cambrica are included in a revised circumscription of Papaver. Three distinct clades are resolved in a combined ITS and trnL–F analysis. Clade 1 consists of Papaver sect. Meconella and Asian Meconopsis. Clade 2 contains a group here identified as Papaver s.s., comprising sections Carinatae, Meconidium, Oxytona, Papaver, Pilosa, Pseudopilosa and Rhoeadium. Clade 3 consists of Papaver sect. Argemonidium and Roemeria refracta. A number of diagnostic indels support these groupings. Within clade 2, sects. Papaver and Rhoeadium are either not monophyletic or lack evidence supporting their monophyly. _ Conclusions The results of this molecular analysis indicate that a number of morphological characters such as valvate capsule dehiscence, dark or light filaments and sessile stigmatic discs have arisen in parallel. The phylogenetic trees are incongruent with the existing taxonomy of Papaver, and a revised classification is suggested.

    Item Type: Article
    Keywords: DNA; Meconopsis; nrITS; Papaver; phylogenetics; Roemeria; Stylomecon; trnL–F;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7170
    Identification Number: https://doi.org/10.1093/aob/mcl079
    Depositing User: James Carolan
    Date Deposited: 07 Jul 2016 11:43
    Journal or Publication Title: Annals of Botany
    Publisher: Oxford University Press
    Refereed: Yes
    URI:

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year