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    Predicted Effector Molecules in the Salivary Secretome of the Pea Aphid (Acyrthosiphon pisum): A Dual Transcriptomic/Proteomic Approach


    Carolan, James C. and Caragea, Doina and Reardon, Karen T. and Mutti, Navdeep S. and Dittmer, Neal and Pappan, Kirk and Cui, Feng and Castaneto, Marisol and Poulain, Julie and Dossat, Carole and Tagu, Denis and Reese, John C. and Reeck, Gerald R. and Wilkinson, Thomas L. and Edwards, Owain R. (2011) Predicted Effector Molecules in the Salivary Secretome of the Pea Aphid (Acyrthosiphon pisum): A Dual Transcriptomic/Proteomic Approach. Journal of Proteome Research, 10 (4). pp. 1505-1518. ISSN 1535-3893

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    Abstract

    The relationship between aphids and their host plants is thought to be functionally analogous to plant-pathogen interactions. Although virulence effector proteins that mediate plant defenses are well-characterized for pathogens such as bacteria, oomycetes, and nematodes, equivalent molecules in aphids and other phloem-feeders are poorly understood. A dual transcriptomic-proteomic approach was adopted to generate a catalog of candidate effector proteins from the salivary glands of the pea aphid, Acyrthosiphon pisum. Of the 1557 transcript supported and 925 mass spectrometry identified proteins, over 300 proteins were identified with secretion signals, including proteins that had previously been identified directly from the secreted saliva. Almost half of the identified proteins have no homologue outside aphids and are of unknown function. Many of the genes encoding the putative effector proteins appear to be evolving at a faster rate than homologues in other insects, and there is strong evidence that genes with multiple copies in the genome are under positive selection. Many of the candidate aphid effector proteins were previously characterized in typical phytopathogenic organisms (e.g., nematodes and fungi) and our results highlight remarkable similarities in the saliva from plant-feeding nematodes and aphids that may indicate the evolution of common solutions to the plant-parasitic lifestyle.

    Item Type: Article
    Additional Information: Supported by grants from the Arthropod Genomics Center (to G.R.R. and C. Culbertson), USDA CREES-NRI (grant G001-35302-00975 to G.R.R. and J.R.R.), the Kansas Agricultural Experiment Station (publication number 11-146-J), Science Foundation Ireland (grant 03/IN3/B381 to T.L.W.), Department of Agriculture Fisheries and Food (RSF07533 to T.L.W.) and an Irish Research Council for Science, Engineering and Technology PhD award to K.R. We thank A. E. Douglas for the provision of the pea aphid image and two anonymous reviewers for their many helpful comments on the manuscript. Access to and use of instrumentation of the UCD Conway Institute Mass Spectrometry Resource is gratefully acknowledged.
    Keywords: Acyrthosiphon pisum; aphids; salivary glands; saliva; effectors;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7171
    Identification Number: dx.doi.org/10.1021/pr100881q
    Depositing User: James Carolan
    Date Deposited: 07 Jul 2016 11:43
    Journal or Publication Title: Journal of Proteome Research
    Publisher: American Chemical Society
    Refereed: Yes
    Funders: Arthropod Genomics Center, USDA CREES-NRI, Kansas Agricultural Experiment Station, Science Foundation Ireland (SFI), Department of Agriculture Fisheries and Food, Irish Research Council for Science Engineering and Technology (IRCSET)
    URI:

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