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    Conditioned media from cell lines: A complementary model to clinical specimens for the discovery of diseasespecific biomarkers


    Dowling, Paul and Clynes, Martin (2011) Conditioned media from cell lines: A complementary model to clinical specimens for the discovery of diseasespecific biomarkers. Proteomics, 11 (4). pp. 794-804. ISSN 1615-9853

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    Abstract

    In the strictest sense, the cell secretome (conditioned media) refers to the collection of proteins that contain a signal peptide and are processed via the endoplasmic reticulum and Golgi apparatus through the classical secretion pathway. More generally, the secretome also encompasses proteins shed from the cell surface and intracellular proteins released through non-classical secretion pathway or exosomes. These secreted proteins include numerous enzymes, growth factors, cytokines and hormones or other soluble mediators. They are fundamental in the processes of cell growth, differentiation, invasion and angiogenesis by regulating cell-to-cell and cell-to-extracellular matrix interactions. The main aim of this review is to provide a synopsis of findings from the analysis of the secretome taking diabetes, cancer and neurodegenerative diseases as examples. We will also discuss the preparation of conditioned media and on the main proteomic-based methodological approaches that have been developed for the study of secreted/shed proteins.

    Item Type: Article
    Keywords: Biomarkers; Biomedicine; Cell culture; Conditioned media; Secretome;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7333
    Identification Number: https://doi.org/10.1002/pmic.201000530
    Depositing User: Paul Dowling
    Date Deposited: 12 Aug 2016 09:41
    Journal or Publication Title: Proteomics
    Publisher: Wiley
    Refereed: Yes
    URI:

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