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    2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time coursedependent remodelling in response to 14 consecutive days of endurance exercise training


    Egan, Brendan and Dowling, Paul and O'Connor, Paul L. and Henry, Michael and Meleady, Paula and Zierath, Juleen R. and O'Gorman, Donal J. (2011) 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time coursedependent remodelling in response to 14 consecutive days of endurance exercise training. Proteomics, 11 (8). pp. 1413-1428. ISSN 1615-9853

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    Abstract

    Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60min at 80% of peak oxygen consumption (VO2peak) each day for 14 consecutive days, resulting in an increase in VO2peak of 17.573.8% (po0.01). Mitochondria-enriched protein fractions from skeletal muscle biopsies taken from m. vastus lateralis at baseline, and on the morning following the 7th and 14th training sessions were subjected to 2-D DIGE analysis with subsequent MS followed by database interrogation to identify the proteins of interest. Thirty-one protein spots were differentially expressed after either 7 or 14 days of training (ANOVA, po0.05). These proteins included subunits of the electron transport chain, enzymes of the tricarboxylic acid cycle, phosphotransfer enzymes, and regulatory factors in mitochondrial protein synthesis, oxygen transport, and antioxidant capacity. Several proteins demonstrated a time course-dependent induction during training. Our results illustrate the phenomenon of skeletal muscle plasticity with the extensive remodelling of the mitochondrial proteome occurring after just 7 days of exercise training suggestive of enhanced capacity for adenosine triphosphate generation at a cellular level.

    Item Type: Article
    Keywords: 2-D DIGE; Biomedicine; Exercise training; Mitochondrial proteome; Skeletal muscle;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7334
    Identification Number: https://doi.org/10.1002/pmic.201000597
    Depositing User: Paul Dowling
    Date Deposited: 12 Aug 2016 09:41
    Journal or Publication Title: Proteomics
    Publisher: Wiley
    Refereed: Yes
    Funders: National Institute for Cellular Biotechnology, Irish Research Council for Science Engineering and Technology (IRCSET), European Foundation for the Study of Diabetes/Novo Nordisk European Clinical Research Programme in Adolescents with Type 2 Diabetes, Strategic Research Program in Diabetes at Karolinska Institutet, Swedish Research Council, European Research Council Advanced Grant Ideas Programme
    URI:

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