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    Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells


    Walsh, Naomi and Dowling, Paul and O'Donovan, Norma and Henry, Michael and Meleady, Paula and Clynes, Martin (2008) Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells. Journal of Proteomics, 71 (5). pp. 561-571. ISSN 1874-3919

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    Abstract

    Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8 (CM#8) inhibited invasion in vitro. 2D DIGE followed by MALDI–TOF MS analysis of CM#3 and CM#8 identified 41 proteins which were differentially regulated; 27 proteins were down-regulated and 14 proteins up-regulated in the invasion-promoting CM#3 when compared to CM#8. Western blotting analysis confirmed the down-regulated expression of gelsolin and the up-regulation of aldehyde dehydrogenase 1A1 in CM#3. Down-regulation of aldehyde dehydrogenase 1A1 in Clone #3 CM and gelsolin levels in Clone #8 CM by siRNA transfection revealed an important involvement of these proteins in promoting and inhibiting invasion in these pancreatic cancer cell lines. Naomi Walsh⁎,1, Paul Dowling1, Norma O'Donovan, Michael Henry, Paula Meleady, Martin Clynes

    Item Type: Article
    Keywords: ALDH1A1; GSN; Pancreatic cancer; Invasion; Conditioned media; 2D DIGE MALDI–TOF MS;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7336
    Identification Number: https://doi.org/10.1016/j.jprot.2008.09.002
    Depositing User: Paul Dowling
    Date Deposited: 12 Aug 2016 15:39
    Journal or Publication Title: Journal of Proteomics
    Publisher: Elsevier
    Refereed: Yes
    Funders: Higher Education Authority (HEA)
    URI:

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