Kumar, Niraj and Maurya, Priyanka and Gammell, Patrick and Dowling, Paul and Clynes, Martin and Meleady, Paula
(2008)
Proteomic Profiling of Secreted Proteins from CHO Cells Using Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry.
Biotechnology Progress, 24 (1).
pp. 273-278.
ISSN 8756-7938
Abstract
Chinese hamster ovary (CHO) cells are the most commonly used host cell line for the production
of recombinant biopharmaceuticals. These biopharmaceuticals are typically secreted from CHO
cells and purified from harvested cell culture media. The purpose of this study was to investigate
changes in the secreted proteome of CHO cells over the various stages of the growth cycle
using Surface Enhanced Laser desorption ionization time-of-flight mass spectrometry (SELDITOF
MS). Conditioned media samples were collected each day over a 6 day growth period
from CHO-K1 cells grown in low serum (0.5% FBS) conditions in monolayer culture. Samples
were profiled on a number of ProteinChip arrays with different chromatographic surfaces. From
this study, 24 proteins were found to be differentially regulated at different phases of the growth
cycle in CHO-K1 cells, when profiled on two chromatographic surfaces, Q10 (anionic) and
IMAC30 (metal affinity) ProteinChip arrays.
| Item Type: |
Article
|
| Keywords: |
Proteomic Profiling; Secreted Proteins; CHO Cells; Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry; SELDITOF MS; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7353 |
| Identification Number: |
https://doi.org/10.1021/bp070244o |
| Depositing User: |
Paul Dowling
|
| Date Deposited: |
17 Aug 2016 15:48 |
| Journal or Publication Title: |
Biotechnology Progress |
| Publisher: |
American Chemical Society and American Institute of Chemical Engineers |
| Refereed: |
Yes |
| Funders: |
Science Foundation Ireland (SFI) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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