Nguyen, Kim-Hong and Jordi, Wilco and Van Dun, Kees and Schepers, Frank
(2008)
Delayed Senescence in Cauliflower Transformed with an Autoregulated Isopentenyl Transferase Gene.
International Journal of Plant Sciences, 169 (3).
pp. 339-347.
ISSN 1058-5893
Abstract
Cauliflower (Brassica oleracea var. botrytis) was transformed, using Agrobacterium tumefaciens, with an
autoregulated isopentenyl transferase (ipt) gene under the control of a senescence-associated gene promoter,
pSAG12, isolated from Arabidopsis thaliana. The effect of introducing this chimeric construct on cytokinin (CK)
content, chlorophyll retention, and plant morphology and development were investigated. A range of CK and
chlorophyll contents was found among the individual primary transformants. Progeny were studied from one of
the primary transformed lines that did not have elevated cytokinin content and was phenotypically similar to the
parent line but displayed delayed leaf senescence. The pSAG12:ipt gene was inherited in a Mendelian manner, and
the effect of this gene on senescence-related parameters was observed in a number of the progeny. While the
pSAG12:ipt progeny did exhibit delayed leaf senescence, it was accompanied by undesirable agronomic traits,
including less synchronous curd initiation, smaller curd size, and greater susceptibility to fungal infection.
| Item Type: |
Article
|
| Keywords: |
Brassica oleracea; cauliflower; cytokinins; isopentnyl adenine; zeatin; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7362 |
| Identification Number: |
https://doi.org/10.1086/526466 |
| Depositing User: |
Prof. Philip J. Dix
|
| Date Deposited: |
23 Aug 2016 10:59 |
| Journal or Publication Title: |
International Journal of Plant Sciences |
| Publisher: |
University of Chicago Press |
| Refereed: |
Yes |
| Funders: |
European Commission |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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