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    Homeologous Plastid DNA Transformation in Tobacco Is Mediated by Multiple Recombination Events


    Kavanagh, Tony A. and Nguyen, Duc Thanh and Lao, Nga Thi and McGrath, Noreen and Peter, Stefan O. and Horvath, Eva and Dix, Philip and Medgyesy, Peter (1999) Homeologous Plastid DNA Transformation in Tobacco Is Mediated by Multiple Recombination Events. Genetics, 152. pp. 1111-1122. ISSN 0016-6731

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    Abstract

    Efficient plastid transformation has been achieved in Nicotiana tabacum using cloned plastid DNA of Solanum nigrum carrying mutations conferring spectinomycin and streptomycin resistance. The use of the incompletely homologous (homeologous) Solanum plastid DNA as donor resulted in a Nicotiana plastid transformation frequency comparable with that of other experiments where completely homologous plastid DNA was introduced. Physical mapping and nucleotide sequence analysis of the targeted plastid DNA region in the transformants demonstrated efficient site-specific integration of the 7.8-kb Solanum plastid DNA and the exclusion of the vector DNA. The integration of the cloned Solanum plastid DNA into the Nicotiana plastid genome involved multiple recombination events as revealed by the presence of discontinuous tracts of Solanum-specific sequences that were interspersed between Nicotiana-specific markers. Marked position effects resulted in very frequent cointegration of the nonselected peripheral donor markers located adjacent to the vector DNA. Data presented here on the efficiency and features of homeologous plastid DNA recombination are consistent with the existence of an active RecA-mediated, but a diminished mismatch, recombination/repair system in higher-plant plastids.

    Item Type: Article
    Keywords: Homeologous; Plastid; DNA; Transformation; Tobacco; Multiple Recombination Events;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7368
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 23 Aug 2016 15:39
    Journal or Publication Title: Genetics
    Publisher: Genetics Society of America
    Refereed: Yes
    URI:

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