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    Over-expression of phenol-oxidising peroxidases alters the UV-susceptibility of transgenic Nicotiana tabacum


    Jansen, Marcel A.K. and Elfstrand, Malin and Heggie, Laura and Sitbon, Folke and Dix, Philip and Thorneley, Roger N.F. (2004) Over-expression of phenol-oxidising peroxidases alters the UV-susceptibility of transgenic Nicotiana tabacum. New Phytologist, 163 (3). pp. 585-594. ISSN 0028-646X

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    Abstract

    * Class III peroxidases catalyse the oxidative crosslinking of UV-absorbing phenolics. The effect of changes in the activity of phenol oxidising peroxidases (EC 1.11.1.7) on UV-tolerance in Nicotiana tabacum plants has been determined. * The UV-sensitivity of transgenic N. tabacum lines, altered in their peroxidase expression pattern, was studied by measuring radiation effects on photosynthetic efficiency. * Analysis of the effect of UV-radiation on the relative variable chlorophyll fluorescence showed that the SPI-2 line, which over-expresses a defence-related cationic peroxidase, is markedly UV-tolerant. By contrast, the ROPN3-line, which overexpresses a synthetic horseradish peroxidase-C gene, was found to be UV-sensitive. The increased activity of indole-3-acetic acid (IAA) inducible peroxidases in homozygous IAA-overproducing transgenic plants was also found to correlate with UV-sensitivity. * It is concluded that only specific peroxidase isozymes, through their effects on phenolic metabolism, contribute to the UV protection response. Thus, the analysis of the role of isozymes in UV-protection addresses fundamental questions of isozyme diversity and/or redundancy in relation to phenolic substrates

    Item Type: Article
    Keywords: UV-radiation; auxin; stress; peroxidase; chlorophyll afluorescence; Nicotiana tabacum; tobacco;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7372
    Identification Number: https://doi.org/10.1111/j.1469-8137.2004.01132.x
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 24 Aug 2016 15:38
    Journal or Publication Title: New Phytologist
    Publisher: Wiley
    Refereed: Yes
    URI:

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