Altpeter, Fredy and Baisakh, Niranjan and Beachy, Roger and Bock, Ralph and Capell, Teresa and Christou, Paul and Daniell, Henry and Datta, Karabi and Datta, Swapan and Dix, Philip and Fauquet, Claude and Huang, Ning and Kohli, Ajay and Mooibroek, Hans and Nicholson, Liz and Nguyen, Thi Thanh and Nugent, Gregory and Raemakers, Krit and Romano, Andrea and Somers, David A. and Stoger, Eva and Taylor, Nigel and Visser, Richard (2005) Particle bombardment and the genetic enhancement of crops: myths and realities. Molecular Breeding, 15 (3). pp. 305-327. ISSN 1380-3743

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Abstract

DNA transfer by particle bombardment makes use of physical processes to achieve the transformation of crop plants. There is no dependence on bacteria, so the limitations inherent in organisms such as Agrobacterium tumefaciens do not apply. The absence of biological constraints, at least until DNA has entered the plant cell, means that particle bombardment is a versatile and effective transformation method, not limited by cell type, species or genotype. There are no intrinsic vector requirements so transgenes of any size and arrangement can be introduced, and multiple gene cotransformation is straightforward. The perceived disadvantages of particle bombardment compared to Agrobacterium-mediated transformation, i.e. the tendency to generate large transgene arrays containing rearranged and broken transgene copies, are not borne out by the recent detailed structural analysis of transgene loci produced by each of the methods. There is also little evidence for major differences in the levels of transgene instability and silencing when these transformation methods are compared in agriculturally important cereals and legumes, and other non-model systems. Indeed, a major advantage of particle bombardment is that the delivered DNA can be manipulated to influence the quality and structure of the resultant transgene loci. This has been demonstrated in recently reported strategies that favor the recovery of transgenic plants containing intact, singlecopy integration events, and demonstrating high-level transgene expression. At the current time, particle bombardment is the most efficient way to achieve plastid transformation in plants and is the only method so far used to achieve mitochondrial transformation. In this review, we discuss recent data highlighting the positive impact of particle bombardment on the genetic transformation of plants, focusing on the fate of exogenous DNA, its organization and its expression in the plant cell. We also discuss some of the most important applications of this technology including the deployment of transgenic plants under field conditions.

Item Type:	Article
Keywords:	Crops; Genetic engineering; Particle bombardment; Transformation; Transgene expression; Transgene structure; Transgenic plants;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7373
Identification Number:	https://doi.org/10.1007/s11032-004-8001-y
Depositing User:	Prof. Philip J. Dix
Date Deposited:	24 Aug 2016 15:38
Journal or Publication Title:	Molecular Breeding
Publisher:	Springer Verlag
Refereed:	Yes
URI:	http://link.springer.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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