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    Transgenic tobacco (Nicotiana tabacum L. cv. Samsun-NN) plants over-expressing a synthetic HRP-C gene are altered in growth, development and susceptibility to abiotic stress


    Heggie, Laura and Jansen, Marcel A.K. and Burbridge, Emma and Kavanagh, Tony A. and Thorneley, Roger N.F. and Dix, Philip (2005) Transgenic tobacco (Nicotiana tabacum L. cv. Samsun-NN) plants over-expressing a synthetic HRP-C gene are altered in growth, development and susceptibility to abiotic stress. Plant Physiology and Biochemistry, 43 (12). pp. 1067-1073. ISSN 0981-9428

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    Abstract

    The physiological role of class III peroxidases (EC 1.11.1.7) in controlling plant growth and development has been investigated by overexpression of both native and heterologous peroxidases. However, it has remained an enigma as to why the phenotypes of different peroxidase over-expressing transgenics vary. In order to resolve the conflicting information about the consequences of peroxidase over-expression, we have explored the role of the subcellular targeting of HRP-C in controlling stem growth, root development, axillary branching and abiotic stress tolerance in tobacco (Nicotiana tabacum L.). Altering the sub-cellular targeting of vacuolar HRP-C, such that over-expressed peroxidase accumulates in the cytoplasm and cell wall, induced phenotypic changes that are typically associated with altered auxin homeostasis, and overexpression of cell wall located peroxidases. We conclude that sub-cellular targeting is a determinant of the phenotype of peroxidase over-expressing plants.

    Item Type: Article
    Keywords: Peroxidase; Isozyme; Nicotiana tabacum; Lignin; Abiotic stress; Auxin;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7385
    Identification Number: https://doi.org/10.1016/j.plaphy.2005.11.002
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 25 Aug 2016 15:49
    Journal or Publication Title: Plant Physiology and Biochemistry
    Publisher: Elsevier
    Refereed: Yes
    Funders: European Commission, Enterprise Ireland (EI)
    URI:

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