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    Optimization of Xylanase Production by Thermomyces Ianuginosus in Solid State Fermentation


    Gaffney, Mark and Doyle, Sean and Murphy, Richard (2009) Optimization of Xylanase Production by Thermomyces Ianuginosus in Solid State Fermentation. Bioscience, Biotechnology, and Biochemistry, 74 (12). pp. 2640-2644. ISSN 0916-8451

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    Abstract

    Extracellular xylanase production by the thermophilic fungus Thermomyces lanuginosus 195 in solid state fermentation (SSF) was found to be significantly affected by fermentation temperature, duration, and inoculum volume (p < or = 0.001). Optimization of these parameters corresponded to a 21.7% increase in xylanase yield. Maximum activity (2,335 U/g of wheat bran) was obtained when 10 g of wheat bran was inoculated with 10 ml of liquid culture and cultivated at 45 degrees C for 40 h. The influence of supplemental carbon and nitrogen sources (3% w/v) on xylanase production was also assessed. Wheat bran, supplemented with glucose and cellulose, facilitated 10% and 7% increases in relative activity respectively. Ammonium based salts, nitrates, and a number of organic nitrogen sources served only to reduce xylanase production (p < or = 0.005) significantly. The enhanced xylanase titers achieved in the present study emphasize the need for optimizing growth conditions for maximum enzyme production in SSF.

    Item Type: Article
    Keywords: xylanase; Thermomyces lanuginosus; optimization; solid state fermentation; SSF; medium supplementation;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7409
    Identification Number: https://doi.org/10.1271/bbb.90493
    Depositing User: Dr. Sean Doyle
    Date Deposited: 29 Aug 2016 15:27
    Journal or Publication Title: Bioscience, Biotechnology, and Biochemistry
    Publisher: Taylor & Francis
    Refereed: Yes
    URI:

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