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    Stable plastid transformation in lettuce (Lactuca sativa L.)


    Lelivelt, Cilia L.C. and McCabe, Matthew S. and Newell, Christine A. and de Snoo, C. Bastiaan and van Dun, Kees M.P. and Birch-Machin, Ian and Gray, John C. and Mills, Kingston H.G. and Nugent, Jacqueline M. (2005) Stable plastid transformation in lettuce (Lactuca sativa L.). Plant Molecular Biology, 58 (6). pp. 763-774. ISSN 0167-4412

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    Abstract

    Although plastid transformation in higher plants was first demonstrated in the early 1990s it is only recently that the technology is being extended to a broader range of species. To date, the production of fertile transplastomic plants has been reported for tobacco, tomato, petunia, soybean, cotton and Lesquerella fendleri (Brassicaceae). In this study we demonstrate a polyethylene glycol-mediated plastid transformation system for lettuce that generates fertile, homoplasmic, plastid-transformed lines. Transformation was achieved using a vector that targets genes to the trnA/trnI intergenic region of the lettuce plastid genome employing the aadA gene as a selectable marker against spectinomycin. Spectinomycin resistance and heterologous gene transcription were shown in T(1) plants derived from self-pollinated primary regenerants demonstrating transmission of the plastid-encoded transgene to the first seed generation. Crossing with male sterile wild-type lettuce showed that spectinomycin resistance was not transmitted via pollen. Constructs containing the gfp gene showed plastid-based expression of green fluorescent protein. The lettuce plastid could have potential both as a production and a delivery system for edible human therapeutic proteins.

    Item Type: Article
    Keywords: aadA; lettuce; PEG; plastid transformationt; rnI/trnA;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7421
    Identification Number: https://doi.org/10.1007/s11103-005-7704-8
    Depositing User: Dr. Jacqueline Nugent
    Date Deposited: 31 Aug 2016 15:11
    Journal or Publication Title: Plant Molecular Biology
    Publisher: Springer Verlag
    Refereed: Yes
    URI:

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